Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24285 and BWI76_RS24290 are separated by 132 nucleotides BWI76_RS24290 and BWI76_RS24295 are separated by 31 nucleotides BWI76_RS24295 and BWI76_RS24300 are separated by 196 nucleotides
BWI76_RS24285: BWI76_RS24285 - cystathionine beta-lyase, at 5,059,322 to 5,060,509
_RS24285
BWI76_RS24290: BWI76_RS24290 - membrane protein, at 5,060,642 to 5,061,301
_RS24290
BWI76_RS24295: BWI76_RS24295 - AraC family transcriptional regulator, at 5,061,333 to 5,062,232
_RS24295
BWI76_RS24300: BWI76_RS24300 - NADH-dependent alcohol dehydrogenase, at 5,062,429 to 5,063,592
_RS24300
Position (kb) 5061
5062
5063 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 5060.592 kb on + strand at 5060.721 kb on + strand, within BWI76_RS24290 at 5060.906 kb on - strand, within BWI76_RS24290 at 5060.949 kb on - strand, within BWI76_RS24290 at 5061.063 kb on + strand, within BWI76_RS24290 at 5061.202 kb on + strand, within BWI76_RS24290 at 5061.347 kb on - strand at 5061.432 kb on - strand, within BWI76_RS24295 at 5061.572 kb on + strand, within BWI76_RS24295 at 5061.662 kb on - strand at 5061.719 kb on + strand, within BWI76_RS24295 at 5061.953 kb on + strand, within BWI76_RS24295 at 5062.013 kb on + strand, within BWI76_RS24295 at 5062.126 kb on - strand, within BWI76_RS24295 at 5062.149 kb on - strand at 5062.252 kb on - strand at 5062.717 kb on + strand, within BWI76_RS24300 at 5062.763 kb on - strand, within BWI76_RS24300 at 5062.973 kb on - strand, within BWI76_RS24300 at 5063.100 kb on + strand, within BWI76_RS24300 at 5063.108 kb on - strand, within BWI76_RS24300 at 5063.214 kb on + strand, within BWI76_RS24300
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 5,060,592 + +0.4 5,060,721 + BWI76_RS24290 0.12 +0.7 5,060,906 - BWI76_RS24290 0.40 +0.1 5,060,949 - BWI76_RS24290 0.47 -0.2 5,061,063 + BWI76_RS24290 0.64 +0.7 5,061,202 + BWI76_RS24290 0.85 -0.1 5,061,347 - -0.2 5,061,432 - BWI76_RS24295 0.11 +0.1 5,061,572 + BWI76_RS24295 0.27 +0.1 5,061,662 - +1.5 5,061,719 + BWI76_RS24295 0.43 +2.2 5,061,953 + BWI76_RS24295 0.69 -0.1 5,062,013 + BWI76_RS24295 0.76 +1.9 5,062,126 - BWI76_RS24295 0.88 +2.7 5,062,149 - -0.5 5,062,252 - +3.9 5,062,717 + BWI76_RS24300 0.25 -0.2 5,062,763 - BWI76_RS24300 0.29 +0.3 5,062,973 - BWI76_RS24300 0.47 -0.4 5,063,100 + BWI76_RS24300 0.58 -1.3 5,063,108 - BWI76_RS24300 0.58 -0.4 5,063,214 + BWI76_RS24300 0.67 -0.2
Or see this region's nucleotide sequence