Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20330

Experiment: KLEB15 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20320 and BWI76_RS20325 are separated by 7 nucleotidesBWI76_RS20325 and BWI76_RS20330 overlap by 4 nucleotidesBWI76_RS20330 and BWI76_RS20335 are separated by 66 nucleotides BWI76_RS20320: BWI76_RS20320 - histidine/lysine/arginine/ornithine ABC transporter ATP-binding protein HisP, at 4,254,772 to 4,255,545 _RS20320 BWI76_RS20325: BWI76_RS20325 - histidine/lysine/arginine/ornithine ABC transporter permease HisM, at 4,255,553 to 4,256,269 _RS20325 BWI76_RS20330: BWI76_RS20330 - histidine/lysine/arginine/ornithine ABC transporter permease HisQ, at 4,256,266 to 4,256,952 _RS20330 BWI76_RS20335: BWI76_RS20335 - zinc transporter, at 4,257,019 to 4,257,801 _RS20335 Position (kb) 4256 4257Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4255.312 kb on - strand, within BWI76_RS20320at 4255.478 kb on + strandat 4255.486 kb on - strandat 4255.487 kb on + strandat 4255.505 kb on + strandat 4255.507 kb on - strandat 4255.824 kb on - strand, within BWI76_RS20325at 4255.893 kb on - strand, within BWI76_RS20325at 4256.054 kb on + strand, within BWI76_RS20325at 4256.074 kb on + strand, within BWI76_RS20325at 4256.271 kb on + strandat 4256.302 kb on - strandat 4256.322 kb on - strandat 4256.360 kb on + strand, within BWI76_RS20330at 4256.425 kb on - strand, within BWI76_RS20330at 4256.449 kb on + strand, within BWI76_RS20330at 4256.722 kb on + strand, within BWI76_RS20330at 4256.802 kb on - strand, within BWI76_RS20330at 4256.898 kb on + strandat 4256.898 kb on + strandat 4256.906 kb on - strandat 4257.586 kb on - strand, within BWI76_RS20335at 4257.598 kb on + strand, within BWI76_RS20335at 4257.680 kb on + strand, within BWI76_RS20335at 4257.705 kb on - strand, within BWI76_RS20335at 4257.814 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 10
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4,255,312 - BWI76_RS20320 0.70 +0.1
4,255,478 + -0.3
4,255,486 - -0.0
4,255,487 + -1.6
4,255,505 + -1.4
4,255,507 - +2.8
4,255,824 - BWI76_RS20325 0.38 -0.1
4,255,893 - BWI76_RS20325 0.47 +0.1
4,256,054 + BWI76_RS20325 0.70 -0.2
4,256,074 + BWI76_RS20325 0.73 +0.9
4,256,271 + -0.7
4,256,302 - -0.3
4,256,322 - +0.2
4,256,360 + BWI76_RS20330 0.14 -0.5
4,256,425 - BWI76_RS20330 0.23 -2.1
4,256,449 + BWI76_RS20330 0.27 -1.2
4,256,722 + BWI76_RS20330 0.66 -3.0
4,256,802 - BWI76_RS20330 0.78 -0.2
4,256,898 + -2.1
4,256,898 + +0.1
4,256,906 - +0.4
4,257,586 - BWI76_RS20335 0.72 +1.0
4,257,598 + BWI76_RS20335 0.74 +1.2
4,257,680 + BWI76_RS20335 0.84 +0.5
4,257,705 - BWI76_RS20335 0.88 -0.1
4,257,814 - -0.7

Or see this region's nucleotide sequence