Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16760

Experiment: KLEB15 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16750 and BWI76_RS16755 are separated by 23 nucleotidesBWI76_RS16755 and BWI76_RS16760 are separated by 57 nucleotidesBWI76_RS16760 and BWI76_RS16765 overlap by 8 nucleotides BWI76_RS16750: BWI76_RS16750 - acetolactate synthase, at 3,469,262 to 3,470,941 _RS16750 BWI76_RS16755: BWI76_RS16755 - acetoin reductase, at 3,470,965 to 3,471,735 _RS16755 BWI76_RS16760: BWI76_RS16760 - lipase, at 3,471,793 to 3,472,863 _RS16760 BWI76_RS16765: BWI76_RS16765 - spermidine/putrescine ABC transporter permease, at 3,472,856 to 3,474,625 _RS16765 Position (kb) 3471 3472 3473Strain fitness (log2 ratio) -2 -1 0 1 2at 3470.925 kb on - strandat 3471.142 kb on - strand, within BWI76_RS16755at 3471.229 kb on - strand, within BWI76_RS16755at 3471.754 kb on - strandat 3471.859 kb on - strandat 3471.966 kb on - strand, within BWI76_RS16760at 3471.970 kb on - strand, within BWI76_RS16760at 3472.087 kb on - strand, within BWI76_RS16760at 3472.494 kb on + strandat 3472.540 kb on - strand, within BWI76_RS16760at 3472.540 kb on - strand, within BWI76_RS16760at 3472.611 kb on + strand, within BWI76_RS16760at 3472.998 kb on + strandat 3473.131 kb on + strand, within BWI76_RS16765at 3473.269 kb on + strand, within BWI76_RS16765at 3473.424 kb on - strand, within BWI76_RS16765

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 10
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3,470,925 - -0.3
3,471,142 - BWI76_RS16755 0.23 +0.3
3,471,229 - BWI76_RS16755 0.34 +0.9
3,471,754 - -1.3
3,471,859 - -0.2
3,471,966 - BWI76_RS16760 0.16 -2.0
3,471,970 - BWI76_RS16760 0.17 -1.7
3,472,087 - BWI76_RS16760 0.27 +0.4
3,472,494 + +1.9
3,472,540 - BWI76_RS16760 0.70 -2.2
3,472,540 - BWI76_RS16760 0.70 +0.1
3,472,611 + BWI76_RS16760 0.76 -1.3
3,472,998 + -0.0
3,473,131 + BWI76_RS16765 0.16 +0.0
3,473,269 + BWI76_RS16765 0.23 -1.4
3,473,424 - BWI76_RS16765 0.32 -1.6

Or see this region's nucleotide sequence