Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16080 and BWI76_RS16085 are separated by 107 nucleotides BWI76_RS16085 and BWI76_RS16090 overlap by 4 nucleotides BWI76_RS16090 and BWI76_RS16095 overlap by 20 nucleotides
BWI76_RS16080: BWI76_RS16080 - LysR family transcriptional regulator, at 3,334,218 to 3,335,114
_RS16080
BWI76_RS16085: BWI76_RS16085 - two-component system sensor histidine kinase DcuS, at 3,335,222 to 3,336,841
_RS16085
BWI76_RS16090: BWI76_RS16090 - two-component system response regulator DcuR, at 3,336,838 to 3,337,557
_RS16090
BWI76_RS16095: BWI76_RS16095 - thiamine biosynthesis protein ApbE, at 3,337,538 to 3,338,488
_RS16095
Position (kb)
3335
3336
3337 Strain fitness (log2 ratio)
-1
0
1
2 at 3334.261 kb on + strand at 3334.356 kb on + strand, within BWI76_RS16080 at 3334.807 kb on - strand, within BWI76_RS16080 at 3334.856 kb on - strand, within BWI76_RS16080 at 3335.359 kb on + strand at 3335.919 kb on + strand, within BWI76_RS16085 at 3335.981 kb on - strand, within BWI76_RS16085 at 3336.249 kb on - strand, within BWI76_RS16085 at 3336.323 kb on - strand, within BWI76_RS16085 at 3336.444 kb on + strand, within BWI76_RS16085 at 3336.452 kb on - strand, within BWI76_RS16085 at 3336.568 kb on + strand, within BWI76_RS16085 at 3336.650 kb on + strand, within BWI76_RS16085 at 3336.822 kb on + strand at 3337.028 kb on - strand, within BWI76_RS16090 at 3337.119 kb on - strand, within BWI76_RS16090 at 3337.143 kb on - strand, within BWI76_RS16090 at 3337.825 kb on + strand, within BWI76_RS16095
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 3,334,261 + -0.0 3,334,356 + BWI76_RS16080 0.15 +0.5 3,334,807 - BWI76_RS16080 0.66 +0.2 3,334,856 - BWI76_RS16080 0.71 +1.9 3,335,359 + -0.1 3,335,919 + BWI76_RS16085 0.43 +2.0 3,335,981 - BWI76_RS16085 0.47 +0.5 3,336,249 - BWI76_RS16085 0.63 -0.4 3,336,323 - BWI76_RS16085 0.68 +1.2 3,336,444 + BWI76_RS16085 0.75 -0.1 3,336,452 - BWI76_RS16085 0.76 +1.8 3,336,568 + BWI76_RS16085 0.83 +0.3 3,336,650 + BWI76_RS16085 0.88 +0.7 3,336,822 + -0.8 3,337,028 - BWI76_RS16090 0.26 +1.1 3,337,119 - BWI76_RS16090 0.39 +2.7 3,337,143 - BWI76_RS16090 0.42 -0.2 3,337,825 + BWI76_RS16095 0.30 +1.4
Or see this region's nucleotide sequence