Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16085

Experiment: KLEB15 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16080 and BWI76_RS16085 are separated by 107 nucleotidesBWI76_RS16085 and BWI76_RS16090 overlap by 4 nucleotidesBWI76_RS16090 and BWI76_RS16095 overlap by 20 nucleotides BWI76_RS16080: BWI76_RS16080 - LysR family transcriptional regulator, at 3,334,218 to 3,335,114 _RS16080 BWI76_RS16085: BWI76_RS16085 - two-component system sensor histidine kinase DcuS, at 3,335,222 to 3,336,841 _RS16085 BWI76_RS16090: BWI76_RS16090 - two-component system response regulator DcuR, at 3,336,838 to 3,337,557 _RS16090 BWI76_RS16095: BWI76_RS16095 - thiamine biosynthesis protein ApbE, at 3,337,538 to 3,338,488 _RS16095 Position (kb) 3335 3336 3337Strain fitness (log2 ratio) -1 0 1 2at 3334.261 kb on + strandat 3334.356 kb on + strand, within BWI76_RS16080at 3334.807 kb on - strand, within BWI76_RS16080at 3334.856 kb on - strand, within BWI76_RS16080at 3335.359 kb on + strandat 3335.919 kb on + strand, within BWI76_RS16085at 3335.981 kb on - strand, within BWI76_RS16085at 3336.249 kb on - strand, within BWI76_RS16085at 3336.323 kb on - strand, within BWI76_RS16085at 3336.444 kb on + strand, within BWI76_RS16085at 3336.452 kb on - strand, within BWI76_RS16085at 3336.568 kb on + strand, within BWI76_RS16085at 3336.650 kb on + strand, within BWI76_RS16085at 3336.822 kb on + strandat 3337.028 kb on - strand, within BWI76_RS16090at 3337.119 kb on - strand, within BWI76_RS16090at 3337.143 kb on - strand, within BWI76_RS16090at 3337.825 kb on + strand, within BWI76_RS16095

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 10
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3,334,261 + -0.0
3,334,356 + BWI76_RS16080 0.15 +0.5
3,334,807 - BWI76_RS16080 0.66 +0.2
3,334,856 - BWI76_RS16080 0.71 +1.9
3,335,359 + -0.1
3,335,919 + BWI76_RS16085 0.43 +2.0
3,335,981 - BWI76_RS16085 0.47 +0.5
3,336,249 - BWI76_RS16085 0.63 -0.4
3,336,323 - BWI76_RS16085 0.68 +1.2
3,336,444 + BWI76_RS16085 0.75 -0.1
3,336,452 - BWI76_RS16085 0.76 +1.8
3,336,568 + BWI76_RS16085 0.83 +0.3
3,336,650 + BWI76_RS16085 0.88 +0.7
3,336,822 + -0.8
3,337,028 - BWI76_RS16090 0.26 +1.1
3,337,119 - BWI76_RS16090 0.39 +2.7
3,337,143 - BWI76_RS16090 0.42 -0.2
3,337,825 + BWI76_RS16095 0.30 +1.4

Or see this region's nucleotide sequence