Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14250 and BWI76_RS14255 overlap by 8 nucleotides BWI76_RS14255 and BWI76_RS14260 overlap by 4 nucleotides BWI76_RS14260 and BWI76_RS14265 are separated by 158 nucleotides
BWI76_RS14250: BWI76_RS14250 - cytochrome ubiquinol oxidase subunit I, at 2,980,363 to 2,981,781
_RS14250
BWI76_RS14255: BWI76_RS14255 - terminal oxidase subunit II, at 2,981,774 to 2,982,778
_RS14255
BWI76_RS14260: BWI76_RS14260 - DUF2474 domain-containing protein, at 2,982,775 to 2,982,930
_RS14260
BWI76_RS14265: BWI76_RS14265 - LysR family transcriptional regulator, at 2,983,089 to 2,984,027
_RS14265
Position (kb)
2981
2982
2983 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2980.778 kb on + strand, within BWI76_RS14250 at 2980.851 kb on - strand, within BWI76_RS14250 at 2980.870 kb on - strand, within BWI76_RS14250 at 2980.872 kb on - strand, within BWI76_RS14250 at 2980.875 kb on + strand, within BWI76_RS14250 at 2980.918 kb on + strand, within BWI76_RS14250 at 2980.918 kb on + strand, within BWI76_RS14250 at 2980.926 kb on - strand, within BWI76_RS14250 at 2980.935 kb on - strand, within BWI76_RS14250 at 2981.037 kb on - strand, within BWI76_RS14250 at 2981.158 kb on - strand, within BWI76_RS14250 at 2981.255 kb on + strand, within BWI76_RS14250 at 2981.255 kb on + strand, within BWI76_RS14250 at 2981.263 kb on - strand at 2981.331 kb on - strand, within BWI76_RS14250 at 2981.362 kb on - strand, within BWI76_RS14250 at 2981.362 kb on - strand, within BWI76_RS14250 at 2981.383 kb on + strand, within BWI76_RS14250 at 2981.565 kb on - strand, within BWI76_RS14250 at 2981.581 kb on - strand, within BWI76_RS14250 at 2981.625 kb on + strand, within BWI76_RS14250 at 2981.635 kb on + strand, within BWI76_RS14250 at 2981.643 kb on - strand at 2981.697 kb on + strand at 2981.726 kb on + strand at 2981.736 kb on + strand at 2981.744 kb on - strand at 2981.824 kb on + strand at 2982.006 kb on + strand, within BWI76_RS14255 at 2982.162 kb on + strand, within BWI76_RS14255 at 2982.170 kb on - strand, within BWI76_RS14255 at 2982.227 kb on - strand, within BWI76_RS14255 at 2982.232 kb on - strand, within BWI76_RS14255 at 2982.253 kb on + strand, within BWI76_RS14255 at 2982.261 kb on - strand, within BWI76_RS14255 at 2982.271 kb on + strand, within BWI76_RS14255 at 2982.297 kb on + strand, within BWI76_RS14255 at 2982.366 kb on - strand, within BWI76_RS14255 at 2982.488 kb on + strand, within BWI76_RS14255 at 2982.488 kb on + strand, within BWI76_RS14255 at 2982.854 kb on - strand, within BWI76_RS14260 at 2982.957 kb on + strand at 2982.974 kb on - strand at 2983.006 kb on - strand at 2983.024 kb on + strand at 2983.032 kb on + strand at 2983.039 kb on - strand at 2983.039 kb on - strand at 2983.055 kb on - strand at 2983.171 kb on + strand at 2983.225 kb on + strand, within BWI76_RS14265 at 2983.372 kb on - strand, within BWI76_RS14265 at 2983.406 kb on - strand, within BWI76_RS14265 at 2983.534 kb on + strand, within BWI76_RS14265 at 2983.636 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.765 kb on - strand, within BWI76_RS14265
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 2,980,778 + BWI76_RS14250 0.29 -0.5 2,980,851 - BWI76_RS14250 0.34 +0.5 2,980,870 - BWI76_RS14250 0.36 -0.8 2,980,872 - BWI76_RS14250 0.36 -1.9 2,980,875 + BWI76_RS14250 0.36 -0.5 2,980,918 + BWI76_RS14250 0.39 -1.6 2,980,918 + BWI76_RS14250 0.39 +0.3 2,980,926 - BWI76_RS14250 0.40 -0.8 2,980,935 - BWI76_RS14250 0.40 -0.8 2,981,037 - BWI76_RS14250 0.47 -0.2 2,981,158 - BWI76_RS14250 0.56 -0.1 2,981,255 + BWI76_RS14250 0.63 -1.1 2,981,255 + BWI76_RS14250 0.63 -0.9 2,981,263 - +1.1 2,981,331 - BWI76_RS14250 0.68 -0.5 2,981,362 - BWI76_RS14250 0.70 -0.4 2,981,362 - BWI76_RS14250 0.70 +0.9 2,981,383 + BWI76_RS14250 0.72 -0.2 2,981,565 - BWI76_RS14250 0.85 -1.6 2,981,581 - BWI76_RS14250 0.86 +0.2 2,981,625 + BWI76_RS14250 0.89 +0.3 2,981,635 + BWI76_RS14250 0.90 -0.4 2,981,643 - +0.0 2,981,697 + -0.3 2,981,726 + +0.2 2,981,736 + -0.5 2,981,744 - -0.3 2,981,824 + -0.1 2,982,006 + BWI76_RS14255 0.23 -4.1 2,982,162 + BWI76_RS14255 0.39 -0.8 2,982,170 - BWI76_RS14255 0.39 -1.7 2,982,227 - BWI76_RS14255 0.45 -0.0 2,982,232 - BWI76_RS14255 0.46 -2.1 2,982,253 + BWI76_RS14255 0.48 +0.7 2,982,261 - BWI76_RS14255 0.48 +0.0 2,982,271 + BWI76_RS14255 0.49 -1.4 2,982,297 + BWI76_RS14255 0.52 +0.5 2,982,366 - BWI76_RS14255 0.59 -2.2 2,982,488 + BWI76_RS14255 0.71 -1.3 2,982,488 + BWI76_RS14255 0.71 -0.1 2,982,854 - BWI76_RS14260 0.51 +1.4 2,982,957 + -0.1 2,982,974 - -0.2 2,983,006 - +0.0 2,983,024 + +0.5 2,983,032 + -0.7 2,983,039 - -0.9 2,983,039 - -0.6 2,983,055 - -0.2 2,983,171 + -1.2 2,983,225 + BWI76_RS14265 0.14 +1.0 2,983,372 - BWI76_RS14265 0.30 +0.5 2,983,406 - BWI76_RS14265 0.34 -0.5 2,983,534 + BWI76_RS14265 0.47 +0.6 2,983,636 + BWI76_RS14265 0.58 +0.1 2,983,757 + BWI76_RS14265 0.71 +2.7 2,983,757 + BWI76_RS14265 0.71 +2.1 2,983,757 + BWI76_RS14265 0.71 +3.1 2,983,765 - BWI76_RS14265 0.72 +1.1
Or see this region's nucleotide sequence