Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14255

Experiment: KLEB15 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14250 and BWI76_RS14255 overlap by 8 nucleotidesBWI76_RS14255 and BWI76_RS14260 overlap by 4 nucleotidesBWI76_RS14260 and BWI76_RS14265 are separated by 158 nucleotides BWI76_RS14250: BWI76_RS14250 - cytochrome ubiquinol oxidase subunit I, at 2,980,363 to 2,981,781 _RS14250 BWI76_RS14255: BWI76_RS14255 - terminal oxidase subunit II, at 2,981,774 to 2,982,778 _RS14255 BWI76_RS14260: BWI76_RS14260 - DUF2474 domain-containing protein, at 2,982,775 to 2,982,930 _RS14260 BWI76_RS14265: BWI76_RS14265 - LysR family transcriptional regulator, at 2,983,089 to 2,984,027 _RS14265 Position (kb) 2981 2982 2983Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2980.778 kb on + strand, within BWI76_RS14250at 2980.851 kb on - strand, within BWI76_RS14250at 2980.870 kb on - strand, within BWI76_RS14250at 2980.872 kb on - strand, within BWI76_RS14250at 2980.875 kb on + strand, within BWI76_RS14250at 2980.918 kb on + strand, within BWI76_RS14250at 2980.918 kb on + strand, within BWI76_RS14250at 2980.926 kb on - strand, within BWI76_RS14250at 2980.935 kb on - strand, within BWI76_RS14250at 2981.037 kb on - strand, within BWI76_RS14250at 2981.158 kb on - strand, within BWI76_RS14250at 2981.255 kb on + strand, within BWI76_RS14250at 2981.255 kb on + strand, within BWI76_RS14250at 2981.263 kb on - strandat 2981.331 kb on - strand, within BWI76_RS14250at 2981.362 kb on - strand, within BWI76_RS14250at 2981.362 kb on - strand, within BWI76_RS14250at 2981.383 kb on + strand, within BWI76_RS14250at 2981.565 kb on - strand, within BWI76_RS14250at 2981.581 kb on - strand, within BWI76_RS14250at 2981.625 kb on + strand, within BWI76_RS14250at 2981.635 kb on + strand, within BWI76_RS14250at 2981.643 kb on - strandat 2981.697 kb on + strandat 2981.726 kb on + strandat 2981.736 kb on + strandat 2981.744 kb on - strandat 2981.824 kb on + strandat 2982.006 kb on + strand, within BWI76_RS14255at 2982.162 kb on + strand, within BWI76_RS14255at 2982.170 kb on - strand, within BWI76_RS14255at 2982.227 kb on - strand, within BWI76_RS14255at 2982.232 kb on - strand, within BWI76_RS14255at 2982.253 kb on + strand, within BWI76_RS14255at 2982.261 kb on - strand, within BWI76_RS14255at 2982.271 kb on + strand, within BWI76_RS14255at 2982.297 kb on + strand, within BWI76_RS14255at 2982.366 kb on - strand, within BWI76_RS14255at 2982.488 kb on + strand, within BWI76_RS14255at 2982.488 kb on + strand, within BWI76_RS14255at 2982.854 kb on - strand, within BWI76_RS14260at 2982.957 kb on + strandat 2982.974 kb on - strandat 2983.006 kb on - strandat 2983.024 kb on + strandat 2983.032 kb on + strandat 2983.039 kb on - strandat 2983.039 kb on - strandat 2983.055 kb on - strandat 2983.171 kb on + strandat 2983.225 kb on + strand, within BWI76_RS14265at 2983.372 kb on - strand, within BWI76_RS14265at 2983.406 kb on - strand, within BWI76_RS14265at 2983.534 kb on + strand, within BWI76_RS14265at 2983.636 kb on + strand, within BWI76_RS14265at 2983.757 kb on + strand, within BWI76_RS14265at 2983.757 kb on + strand, within BWI76_RS14265at 2983.757 kb on + strand, within BWI76_RS14265at 2983.765 kb on - strand, within BWI76_RS14265

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 10
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2,980,778 + BWI76_RS14250 0.29 -0.5
2,980,851 - BWI76_RS14250 0.34 +0.5
2,980,870 - BWI76_RS14250 0.36 -0.8
2,980,872 - BWI76_RS14250 0.36 -1.9
2,980,875 + BWI76_RS14250 0.36 -0.5
2,980,918 + BWI76_RS14250 0.39 -1.6
2,980,918 + BWI76_RS14250 0.39 +0.3
2,980,926 - BWI76_RS14250 0.40 -0.8
2,980,935 - BWI76_RS14250 0.40 -0.8
2,981,037 - BWI76_RS14250 0.47 -0.2
2,981,158 - BWI76_RS14250 0.56 -0.1
2,981,255 + BWI76_RS14250 0.63 -1.1
2,981,255 + BWI76_RS14250 0.63 -0.9
2,981,263 - +1.1
2,981,331 - BWI76_RS14250 0.68 -0.5
2,981,362 - BWI76_RS14250 0.70 -0.4
2,981,362 - BWI76_RS14250 0.70 +0.9
2,981,383 + BWI76_RS14250 0.72 -0.2
2,981,565 - BWI76_RS14250 0.85 -1.6
2,981,581 - BWI76_RS14250 0.86 +0.2
2,981,625 + BWI76_RS14250 0.89 +0.3
2,981,635 + BWI76_RS14250 0.90 -0.4
2,981,643 - +0.0
2,981,697 + -0.3
2,981,726 + +0.2
2,981,736 + -0.5
2,981,744 - -0.3
2,981,824 + -0.1
2,982,006 + BWI76_RS14255 0.23 -4.1
2,982,162 + BWI76_RS14255 0.39 -0.8
2,982,170 - BWI76_RS14255 0.39 -1.7
2,982,227 - BWI76_RS14255 0.45 -0.0
2,982,232 - BWI76_RS14255 0.46 -2.1
2,982,253 + BWI76_RS14255 0.48 +0.7
2,982,261 - BWI76_RS14255 0.48 +0.0
2,982,271 + BWI76_RS14255 0.49 -1.4
2,982,297 + BWI76_RS14255 0.52 +0.5
2,982,366 - BWI76_RS14255 0.59 -2.2
2,982,488 + BWI76_RS14255 0.71 -1.3
2,982,488 + BWI76_RS14255 0.71 -0.1
2,982,854 - BWI76_RS14260 0.51 +1.4
2,982,957 + -0.1
2,982,974 - -0.2
2,983,006 - +0.0
2,983,024 + +0.5
2,983,032 + -0.7
2,983,039 - -0.9
2,983,039 - -0.6
2,983,055 - -0.2
2,983,171 + -1.2
2,983,225 + BWI76_RS14265 0.14 +1.0
2,983,372 - BWI76_RS14265 0.30 +0.5
2,983,406 - BWI76_RS14265 0.34 -0.5
2,983,534 + BWI76_RS14265 0.47 +0.6
2,983,636 + BWI76_RS14265 0.58 +0.1
2,983,757 + BWI76_RS14265 0.71 +2.7
2,983,757 + BWI76_RS14265 0.71 +2.1
2,983,757 + BWI76_RS14265 0.71 +3.1
2,983,765 - BWI76_RS14265 0.72 +1.1

Or see this region's nucleotide sequence