Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14245

Experiment: KLEB15 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14235 and BWI76_RS14240 are separated by 43 nucleotidesBWI76_RS14240 and BWI76_RS14245 are separated by 35 nucleotidesBWI76_RS14245 and BWI76_RS14250 are separated by 111 nucleotides BWI76_RS14235: BWI76_RS14235 - alcohol dehydrogenase, at 2,978,303 to 2,979,327 _RS14235 BWI76_RS14240: BWI76_RS14240 - thiol reductase thioredoxin, at 2,979,371 to 2,979,694 _RS14240 BWI76_RS14245: BWI76_RS14245 - carboxymuconolactone decarboxylase family protein, at 2,979,730 to 2,980,251 _RS14245 BWI76_RS14250: BWI76_RS14250 - cytochrome ubiquinol oxidase subunit I, at 2,980,363 to 2,981,781 _RS14250 Position (kb) 2979 2980 2981Strain fitness (log2 ratio) -2 -1 0 1 2at 2978.744 kb on + strand, within BWI76_RS14235at 2978.892 kb on + strand, within BWI76_RS14235at 2978.900 kb on - strand, within BWI76_RS14235at 2978.940 kb on + strand, within BWI76_RS14235at 2979.367 kb on + strandat 2979.468 kb on - strand, within BWI76_RS14240at 2979.563 kb on - strand, within BWI76_RS14240at 2979.626 kb on + strand, within BWI76_RS14240at 2979.634 kb on - strand, within BWI76_RS14240at 2979.743 kb on + strandat 2979.796 kb on + strand, within BWI76_RS14245at 2979.926 kb on + strand, within BWI76_RS14245at 2979.943 kb on - strand, within BWI76_RS14245at 2979.990 kb on - strand, within BWI76_RS14245at 2980.261 kb on + strandat 2980.330 kb on - strandat 2980.581 kb on + strandat 2980.589 kb on - strand, within BWI76_RS14250at 2980.703 kb on + strand, within BWI76_RS14250at 2980.711 kb on - strand, within BWI76_RS14250at 2980.778 kb on + strand, within BWI76_RS14250at 2980.851 kb on - strand, within BWI76_RS14250at 2980.870 kb on - strand, within BWI76_RS14250at 2980.872 kb on - strand, within BWI76_RS14250at 2980.875 kb on + strand, within BWI76_RS14250at 2980.918 kb on + strand, within BWI76_RS14250at 2980.918 kb on + strand, within BWI76_RS14250at 2980.926 kb on - strand, within BWI76_RS14250at 2980.935 kb on - strand, within BWI76_RS14250at 2981.037 kb on - strand, within BWI76_RS14250at 2981.158 kb on - strand, within BWI76_RS14250

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 10
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2,978,744 + BWI76_RS14235 0.43 -0.9
2,978,892 + BWI76_RS14235 0.57 -0.2
2,978,900 - BWI76_RS14235 0.58 -0.3
2,978,940 + BWI76_RS14235 0.62 +0.6
2,979,367 + -0.4
2,979,468 - BWI76_RS14240 0.30 -0.8
2,979,563 - BWI76_RS14240 0.59 +0.9
2,979,626 + BWI76_RS14240 0.79 -0.8
2,979,634 - BWI76_RS14240 0.81 +0.2
2,979,743 + +1.4
2,979,796 + BWI76_RS14245 0.13 -1.5
2,979,926 + BWI76_RS14245 0.38 -2.0
2,979,943 - BWI76_RS14245 0.41 -1.2
2,979,990 - BWI76_RS14245 0.50 +0.5
2,980,261 + +0.1
2,980,330 - -0.5
2,980,581 + +2.1
2,980,589 - BWI76_RS14250 0.16 +0.2
2,980,703 + BWI76_RS14250 0.24 -0.4
2,980,711 - BWI76_RS14250 0.25 -0.1
2,980,778 + BWI76_RS14250 0.29 -0.5
2,980,851 - BWI76_RS14250 0.34 +0.5
2,980,870 - BWI76_RS14250 0.36 -0.8
2,980,872 - BWI76_RS14250 0.36 -1.9
2,980,875 + BWI76_RS14250 0.36 -0.5
2,980,918 + BWI76_RS14250 0.39 -1.6
2,980,918 + BWI76_RS14250 0.39 +0.3
2,980,926 - BWI76_RS14250 0.40 -0.8
2,980,935 - BWI76_RS14250 0.40 -0.8
2,981,037 - BWI76_RS14250 0.47 -0.2
2,981,158 - BWI76_RS14250 0.56 -0.1

Or see this region's nucleotide sequence