Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12815 and BWI76_RS12820 are separated by 235 nucleotides BWI76_RS12820 and BWI76_RS12825 are separated by 131 nucleotides BWI76_RS12825 and BWI76_RS12830 are separated by 4 nucleotides
BWI76_RS12815: BWI76_RS12815 - transcriptional regulator FeaR, at 2,670,113 to 2,671,021
_RS12815
BWI76_RS12820: BWI76_RS12820 - NAD-dependent phenylacetaldehyde dehydrogenase, at 2,671,257 to 2,672,756
_RS12820
BWI76_RS12825: BWI76_RS12825 - hypothetical protein, at 2,672,888 to 2,673,619
_RS12825
BWI76_RS12830: BWI76_RS12830 - flagellar motor switch protein FliY, at 2,673,624 to 2,674,454
_RS12830
Position (kb)
2671
2672
2673 Strain fitness (log2 ratio)
-1
0
1
2 at 2670.529 kb on + strand, within BWI76_RS12815 at 2670.614 kb on + strand, within BWI76_RS12815 at 2671.231 kb on - strand at 2671.242 kb on + strand at 2671.441 kb on + strand, within BWI76_RS12820 at 2671.485 kb on + strand, within BWI76_RS12820 at 2671.493 kb on - strand, within BWI76_RS12820 at 2671.581 kb on - strand, within BWI76_RS12820 at 2671.609 kb on + strand at 2671.970 kb on - strand, within BWI76_RS12820 at 2672.084 kb on + strand, within BWI76_RS12820 at 2672.296 kb on - strand, within BWI76_RS12820 at 2672.415 kb on - strand, within BWI76_RS12820 at 2672.527 kb on - strand, within BWI76_RS12820 at 2672.640 kb on - strand at 2672.640 kb on - strand at 2672.899 kb on - strand at 2673.302 kb on + strand, within BWI76_RS12825
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 2,670,529 + BWI76_RS12815 0.46 -0.2 2,670,614 + BWI76_RS12815 0.55 +0.0 2,671,231 - +1.0 2,671,242 + -0.9 2,671,441 + BWI76_RS12820 0.12 -0.4 2,671,485 + BWI76_RS12820 0.15 +0.5 2,671,493 - BWI76_RS12820 0.16 -1.0 2,671,581 - BWI76_RS12820 0.22 +1.0 2,671,609 + +2.0 2,671,970 - BWI76_RS12820 0.48 +0.5 2,672,084 + BWI76_RS12820 0.55 +0.8 2,672,296 - BWI76_RS12820 0.69 +1.3 2,672,415 - BWI76_RS12820 0.77 +1.8 2,672,527 - BWI76_RS12820 0.85 -0.1 2,672,640 - +2.0 2,672,640 - -0.1 2,672,899 - -0.1 2,673,302 + BWI76_RS12825 0.57 +0.2
Or see this region's nucleotide sequence