Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11240

Experiment: KLEB15 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11230 and BWI76_RS11235 are separated by 28 nucleotidesBWI76_RS11235 and BWI76_RS11240 are separated by 346 nucleotidesBWI76_RS11240 and BWI76_RS11245 overlap by 4 nucleotidesBWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotides BWI76_RS11230: BWI76_RS11230 - NAD-dependent deacylase, at 2,369,681 to 2,370,526 _RS11230 BWI76_RS11235: BWI76_RS11235 - hypothetical protein, at 2,370,555 to 2,370,860 _RS11235 BWI76_RS11240: BWI76_RS11240 - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 2,371,207 to 2,372,253 _RS11240 BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035 _RS11245 BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889 _RS11250 Position (kb) 2371 2372 2373Strain fitness (log2 ratio) -2 -1 0 1at 2370.211 kb on - strand, within BWI76_RS11230at 2370.335 kb on - strand, within BWI76_RS11230at 2370.442 kb on + strandat 2370.552 kb on + strandat 2370.560 kb on - strandat 2370.623 kb on + strand, within BWI76_RS11235at 2370.904 kb on + strandat 2371.030 kb on + strandat 2371.166 kb on - strandat 2371.571 kb on + strand, within BWI76_RS11240at 2371.715 kb on + strand, within BWI76_RS11240at 2371.725 kb on + strand, within BWI76_RS11240at 2371.762 kb on - strand, within BWI76_RS11240at 2371.867 kb on - strand, within BWI76_RS11240at 2372.461 kb on - strand, within BWI76_RS11245at 2372.599 kb on - strand, within BWI76_RS11245at 2372.922 kb on + strand, within BWI76_RS11245

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 10
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2,370,211 - BWI76_RS11230 0.63 -0.6
2,370,335 - BWI76_RS11230 0.77 +1.1
2,370,442 + -0.0
2,370,552 + +0.7
2,370,560 - +0.4
2,370,623 + BWI76_RS11235 0.22 +0.3
2,370,904 + +0.7
2,371,030 + +0.7
2,371,166 - +0.2
2,371,571 + BWI76_RS11240 0.35 -2.2
2,371,715 + BWI76_RS11240 0.49 -0.5
2,371,725 + BWI76_RS11240 0.49 -2.5
2,371,762 - BWI76_RS11240 0.53 -0.5
2,371,867 - BWI76_RS11240 0.63 -0.5
2,372,461 - BWI76_RS11245 0.27 -0.7
2,372,599 - BWI76_RS11245 0.44 +0.2
2,372,922 + BWI76_RS11245 0.85 -0.4

Or see this region's nucleotide sequence