Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08270 and BWI76_RS08275 are separated by 50 nucleotides BWI76_RS08275 and BWI76_RS08280 are separated by 133 nucleotides BWI76_RS08280 and BWI76_RS08285 are separated by 184 nucleotides BWI76_RS08285 and BWI76_RS08290 are separated by 32 nucleotides
BWI76_RS08270: BWI76_RS08270 - flavodoxin, at 1,750,879 to 1,751,508
_RS08270
BWI76_RS08275: BWI76_RS08275 - LexA regulated protein, at 1,751,559 to 1,751,852
_RS08275
BWI76_RS08280: BWI76_RS08280 - acyl-CoA esterase, at 1,751,986 to 1,752,759
_RS08280
BWI76_RS08285: BWI76_RS08285 - replication initiation regulator SeqA, at 1,752,944 to 1,753,492
_RS08285
BWI76_RS08290: BWI76_RS08290 - phosphoglucomutase, alpha-D-glucose phosphate-specific, at 1,753,525 to 1,755,165
_RS08290
Position (kb)
1751
1752
1753 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1751.518 kb on + strand at 1751.526 kb on - strand at 1751.571 kb on - strand at 1751.722 kb on + strand, within BWI76_RS08275 at 1751.754 kb on - strand, within BWI76_RS08275 at 1752.008 kb on - strand at 1752.163 kb on - strand, within BWI76_RS08280 at 1752.589 kb on + strand, within BWI76_RS08280 at 1752.605 kb on - strand, within BWI76_RS08280 at 1752.857 kb on + strand at 1752.862 kb on + strand at 1752.936 kb on + strand at 1753.237 kb on + strand, within BWI76_RS08285 at 1753.371 kb on - strand, within BWI76_RS08285
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 1,751,518 + -1.2 1,751,526 - -0.3 1,751,571 - +0.8 1,751,722 + BWI76_RS08275 0.55 +1.5 1,751,754 - BWI76_RS08275 0.66 -1.7 1,752,008 - -0.2 1,752,163 - BWI76_RS08280 0.23 -0.6 1,752,589 + BWI76_RS08280 0.78 -0.2 1,752,605 - BWI76_RS08280 0.80 -2.5 1,752,857 + -0.4 1,752,862 + -0.2 1,752,936 + +0.1 1,753,237 + BWI76_RS08285 0.53 -0.2 1,753,371 - BWI76_RS08285 0.78 +1.8
Or see this region's nucleotide sequence