Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05050 and BWI76_RS05055 overlap by 8 nucleotides BWI76_RS05055 and BWI76_RS05060 overlap by 4 nucleotides BWI76_RS05060 and BWI76_RS05065 overlap by 4 nucleotides BWI76_RS05065 and BWI76_RS05070 overlap by 4 nucleotides
BWI76_RS05050: BWI76_RS05050 - general secretion pathway protein GspL, at 1,058,509 to 1,059,705
_RS05050
BWI76_RS05055: BWI76_RS05055 - type II secretion system protein K, at 1,059,698 to 1,060,678
_RS05055
BWI76_RS05060: BWI76_RS05060 - type II secretion system protein GspJ, at 1,060,675 to 1,061,271
_RS05060
BWI76_RS05065: BWI76_RS05065 - type II secretion system protein GspI, at 1,061,268 to 1,061,633
_RS05065
BWI76_RS05070: BWI76_RS05070 - type II secretion system protein GspH, at 1,061,630 to 1,062,142
_RS05070
Position (kb)
1059
1060
1061 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1059.018 kb on + strand, within BWI76_RS05050 at 1059.306 kb on + strand, within BWI76_RS05050 at 1059.306 kb on + strand, within BWI76_RS05050 at 1059.533 kb on - strand, within BWI76_RS05050 at 1059.797 kb on + strand, within BWI76_RS05055 at 1059.970 kb on + strand, within BWI76_RS05055 at 1060.020 kb on + strand, within BWI76_RS05055 at 1060.020 kb on + strand, within BWI76_RS05055 at 1060.020 kb on + strand, within BWI76_RS05055 at 1060.106 kb on - strand, within BWI76_RS05055 at 1060.181 kb on - strand, within BWI76_RS05055 at 1060.242 kb on + strand, within BWI76_RS05055 at 1060.267 kb on + strand, within BWI76_RS05055 at 1060.311 kb on + strand, within BWI76_RS05055 at 1060.348 kb on + strand, within BWI76_RS05055 at 1060.371 kb on - strand, within BWI76_RS05055 at 1060.438 kb on + strand at 1060.445 kb on + strand, within BWI76_RS05055 at 1060.453 kb on - strand, within BWI76_RS05055 at 1060.607 kb on + strand at 1060.649 kb on - strand at 1060.776 kb on + strand, within BWI76_RS05060 at 1060.835 kb on + strand, within BWI76_RS05060 at 1060.850 kb on - strand, within BWI76_RS05060 at 1060.850 kb on - strand, within BWI76_RS05060 at 1060.850 kb on - strand, within BWI76_RS05060 at 1060.854 kb on + strand, within BWI76_RS05060 at 1060.879 kb on + strand, within BWI76_RS05060 at 1060.979 kb on - strand, within BWI76_RS05060 at 1061.022 kb on + strand, within BWI76_RS05060 at 1061.244 kb on + strand at 1061.244 kb on + strand at 1061.244 kb on + strand at 1061.252 kb on - strand at 1061.451 kb on - strand, within BWI76_RS05065
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 1,059,018 + BWI76_RS05050 0.43 -2.4 1,059,306 + BWI76_RS05050 0.67 +2.3 1,059,306 + BWI76_RS05050 0.67 -1.6 1,059,533 - BWI76_RS05050 0.86 +1.0 1,059,797 + BWI76_RS05055 0.10 +0.8 1,059,970 + BWI76_RS05055 0.28 +0.4 1,060,020 + BWI76_RS05055 0.33 +0.8 1,060,020 + BWI76_RS05055 0.33 -0.0 1,060,020 + BWI76_RS05055 0.33 +1.5 1,060,106 - BWI76_RS05055 0.42 +0.3 1,060,181 - BWI76_RS05055 0.49 +0.3 1,060,242 + BWI76_RS05055 0.55 +1.1 1,060,267 + BWI76_RS05055 0.58 -0.2 1,060,311 + BWI76_RS05055 0.62 +0.8 1,060,348 + BWI76_RS05055 0.66 +0.2 1,060,371 - BWI76_RS05055 0.69 +0.7 1,060,438 + +1.8 1,060,445 + BWI76_RS05055 0.76 +1.8 1,060,453 - BWI76_RS05055 0.77 +0.7 1,060,607 + -0.1 1,060,649 - -0.2 1,060,776 + BWI76_RS05060 0.17 -1.1 1,060,835 + BWI76_RS05060 0.27 +0.1 1,060,850 - BWI76_RS05060 0.29 +2.4 1,060,850 - BWI76_RS05060 0.29 +0.7 1,060,850 - BWI76_RS05060 0.29 +0.1 1,060,854 + BWI76_RS05060 0.30 +0.1 1,060,879 + BWI76_RS05060 0.34 +0.2 1,060,979 - BWI76_RS05060 0.51 +0.4 1,061,022 + BWI76_RS05060 0.58 +0.8 1,061,244 + -0.8 1,061,244 + +0.4 1,061,244 + +0.4 1,061,252 - -0.7 1,061,451 - BWI76_RS05065 0.50 -0.3
Or see this region's nucleotide sequence