Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05055

Experiment: KLEB15 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05050 and BWI76_RS05055 overlap by 8 nucleotidesBWI76_RS05055 and BWI76_RS05060 overlap by 4 nucleotidesBWI76_RS05060 and BWI76_RS05065 overlap by 4 nucleotidesBWI76_RS05065 and BWI76_RS05070 overlap by 4 nucleotides BWI76_RS05050: BWI76_RS05050 - general secretion pathway protein GspL, at 1,058,509 to 1,059,705 _RS05050 BWI76_RS05055: BWI76_RS05055 - type II secretion system protein K, at 1,059,698 to 1,060,678 _RS05055 BWI76_RS05060: BWI76_RS05060 - type II secretion system protein GspJ, at 1,060,675 to 1,061,271 _RS05060 BWI76_RS05065: BWI76_RS05065 - type II secretion system protein GspI, at 1,061,268 to 1,061,633 _RS05065 BWI76_RS05070: BWI76_RS05070 - type II secretion system protein GspH, at 1,061,630 to 1,062,142 _RS05070 Position (kb) 1059 1060 1061Strain fitness (log2 ratio) -2 -1 0 1 2at 1059.018 kb on + strand, within BWI76_RS05050at 1059.306 kb on + strand, within BWI76_RS05050at 1059.306 kb on + strand, within BWI76_RS05050at 1059.533 kb on - strand, within BWI76_RS05050at 1059.797 kb on + strand, within BWI76_RS05055at 1059.970 kb on + strand, within BWI76_RS05055at 1060.020 kb on + strand, within BWI76_RS05055at 1060.020 kb on + strand, within BWI76_RS05055at 1060.020 kb on + strand, within BWI76_RS05055at 1060.106 kb on - strand, within BWI76_RS05055at 1060.181 kb on - strand, within BWI76_RS05055at 1060.242 kb on + strand, within BWI76_RS05055at 1060.267 kb on + strand, within BWI76_RS05055at 1060.311 kb on + strand, within BWI76_RS05055at 1060.348 kb on + strand, within BWI76_RS05055at 1060.371 kb on - strand, within BWI76_RS05055at 1060.438 kb on + strandat 1060.445 kb on + strand, within BWI76_RS05055at 1060.453 kb on - strand, within BWI76_RS05055at 1060.607 kb on + strandat 1060.649 kb on - strandat 1060.776 kb on + strand, within BWI76_RS05060at 1060.835 kb on + strand, within BWI76_RS05060at 1060.850 kb on - strand, within BWI76_RS05060at 1060.850 kb on - strand, within BWI76_RS05060at 1060.850 kb on - strand, within BWI76_RS05060at 1060.854 kb on + strand, within BWI76_RS05060at 1060.879 kb on + strand, within BWI76_RS05060at 1060.979 kb on - strand, within BWI76_RS05060at 1061.022 kb on + strand, within BWI76_RS05060at 1061.244 kb on + strandat 1061.244 kb on + strandat 1061.244 kb on + strandat 1061.252 kb on - strandat 1061.451 kb on - strand, within BWI76_RS05065

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 10
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1,059,018 + BWI76_RS05050 0.43 -2.4
1,059,306 + BWI76_RS05050 0.67 +2.3
1,059,306 + BWI76_RS05050 0.67 -1.6
1,059,533 - BWI76_RS05050 0.86 +1.0
1,059,797 + BWI76_RS05055 0.10 +0.8
1,059,970 + BWI76_RS05055 0.28 +0.4
1,060,020 + BWI76_RS05055 0.33 +0.8
1,060,020 + BWI76_RS05055 0.33 -0.0
1,060,020 + BWI76_RS05055 0.33 +1.5
1,060,106 - BWI76_RS05055 0.42 +0.3
1,060,181 - BWI76_RS05055 0.49 +0.3
1,060,242 + BWI76_RS05055 0.55 +1.1
1,060,267 + BWI76_RS05055 0.58 -0.2
1,060,311 + BWI76_RS05055 0.62 +0.8
1,060,348 + BWI76_RS05055 0.66 +0.2
1,060,371 - BWI76_RS05055 0.69 +0.7
1,060,438 + +1.8
1,060,445 + BWI76_RS05055 0.76 +1.8
1,060,453 - BWI76_RS05055 0.77 +0.7
1,060,607 + -0.1
1,060,649 - -0.2
1,060,776 + BWI76_RS05060 0.17 -1.1
1,060,835 + BWI76_RS05060 0.27 +0.1
1,060,850 - BWI76_RS05060 0.29 +2.4
1,060,850 - BWI76_RS05060 0.29 +0.7
1,060,850 - BWI76_RS05060 0.29 +0.1
1,060,854 + BWI76_RS05060 0.30 +0.1
1,060,879 + BWI76_RS05060 0.34 +0.2
1,060,979 - BWI76_RS05060 0.51 +0.4
1,061,022 + BWI76_RS05060 0.58 +0.8
1,061,244 + -0.8
1,061,244 + +0.4
1,061,244 + +0.4
1,061,252 - -0.7
1,061,451 - BWI76_RS05065 0.50 -0.3

Or see this region's nucleotide sequence