Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01315 and BWI76_RS01320 are separated by 76 nucleotides BWI76_RS01320 and BWI76_RS01325 are separated by 3 nucleotides BWI76_RS01325 and BWI76_RS01330 are separated by 2 nucleotides BWI76_RS01330 and BWI76_RS01335 are separated by 42 nucleotides BWI76_RS01335 and BWI76_RS01340 are separated by 6 nucleotides
BWI76_RS01315: BWI76_RS01315 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 269,971 to 271,611
_RS01315
BWI76_RS01320: BWI76_RS01320 - twin-arginine translocase subunit TatA, at 271,688 to 271,939
_RS01320
BWI76_RS01325: BWI76_RS01325 - twin-arginine translocase subunit TatB, at 271,943 to 272,482
_RS01325
BWI76_RS01330: BWI76_RS01330 - twin-arginine translocase subunit TatC, at 272,485 to 273,258
_RS01330
BWI76_RS01335: BWI76_RS01335 - DNase TatD, at 273,301 to 274,083
_RS01335
BWI76_RS01340: BWI76_RS01340 - transcription/translation regulatory transformer protein RfaH, at 274,090 to 274,578
_RS01340
Position (kb) 272
273
274 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 271.595 kb on + strand at 271.692 kb on - strand at 271.933 kb on + strand at 272.135 kb on - strand, within BWI76_RS01325 at 272.158 kb on - strand, within BWI76_RS01325 at 272.347 kb on - strand, within BWI76_RS01325 at 272.522 kb on + strand at 272.781 kb on + strand, within BWI76_RS01330 at 272.796 kb on - strand, within BWI76_RS01330 at 272.822 kb on + strand, within BWI76_RS01330 at 272.835 kb on + strand, within BWI76_RS01330 at 272.915 kb on + strand, within BWI76_RS01330 at 273.194 kb on - strand at 273.210 kb on - strand at 273.253 kb on + strand at 273.358 kb on + strand at 273.407 kb on + strand, within BWI76_RS01335 at 273.479 kb on + strand, within BWI76_RS01335 at 273.615 kb on - strand, within BWI76_RS01335 at 273.799 kb on - strand, within BWI76_RS01335 at 273.848 kb on + strand, within BWI76_RS01335 at 273.985 kb on + strand, within BWI76_RS01335 at 274.028 kb on + strand at 274.028 kb on - strand at 274.036 kb on - strand at 274.123 kb on + strand at 274.123 kb on + strand at 274.123 kb on + strand at 274.123 kb on + strand at 274.131 kb on - strand at 274.131 kb on - strand at 274.131 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 271,595 + +0.1 271,692 - -0.7 271,933 + +0.8 272,135 - BWI76_RS01325 0.36 -1.6 272,158 - BWI76_RS01325 0.40 -1.8 272,347 - BWI76_RS01325 0.75 -4.0 272,522 + +2.6 272,781 + BWI76_RS01330 0.38 -3.3 272,796 - BWI76_RS01330 0.40 +0.0 272,822 + BWI76_RS01330 0.44 -3.0 272,835 + BWI76_RS01330 0.45 +0.6 272,915 + BWI76_RS01330 0.56 +6.0 273,194 - -0.4 273,210 - -0.2 273,253 + +0.8 273,358 + +0.1 273,407 + BWI76_RS01335 0.14 +2.6 273,479 + BWI76_RS01335 0.23 +1.2 273,615 - BWI76_RS01335 0.40 -0.2 273,799 - BWI76_RS01335 0.64 +0.4 273,848 + BWI76_RS01335 0.70 -0.0 273,985 + BWI76_RS01335 0.87 -0.6 274,028 + -0.5 274,028 - +0.1 274,036 - -0.2 274,123 + -1.8 274,123 + -0.1 274,123 + -0.3 274,123 + -0.6 274,131 - -1.2 274,131 - -0.2 274,131 - +0.5
Or see this region's nucleotide sequence