Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00715 and BWI76_RS00720 overlap by 4 nucleotides BWI76_RS00720 and BWI76_RS00725 are separated by 149 nucleotides BWI76_RS00725 and BWI76_RS00730 are separated by 149 nucleotides
BWI76_RS00715: BWI76_RS00715 - two-component sensor histidine kinase, at 145,529 to 146,902
_RS00715
BWI76_RS00720: BWI76_RS00720 - DNA-binding response regulator, at 146,899 to 147,597
_RS00720
BWI76_RS00725: BWI76_RS00725 - periplasmic repressor CpxP, at 147,747 to 148,250
_RS00725
BWI76_RS00730: BWI76_RS00730 - cation-efflux pump FieF, at 148,400 to 149,296
_RS00730
Position (kb) 146
147
148 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 146.062 kb on + strand, within BWI76_RS00715 at 146.068 kb on + strand, within BWI76_RS00715 at 146.146 kb on - strand at 146.232 kb on - strand, within BWI76_RS00715 at 146.232 kb on - strand, within BWI76_RS00715 at 146.345 kb on - strand, within BWI76_RS00715 at 146.348 kb on + strand, within BWI76_RS00715 at 146.554 kb on + strand, within BWI76_RS00715 at 147.037 kb on + strand, within BWI76_RS00720 at 147.129 kb on + strand, within BWI76_RS00720 at 147.356 kb on - strand, within BWI76_RS00720 at 147.524 kb on - strand, within BWI76_RS00720 at 147.661 kb on + strand at 147.669 kb on - strand at 147.669 kb on - strand at 147.861 kb on - strand, within BWI76_RS00725 at 147.897 kb on - strand, within BWI76_RS00725 at 148.215 kb on + strand at 148.368 kb on + strand at 148.411 kb on - strand at 148.416 kb on - strand at 148.431 kb on - strand at 148.453 kb on + strand at 148.500 kb on - strand, within BWI76_RS00730 at 148.503 kb on - strand, within BWI76_RS00730 at 148.575 kb on - strand, within BWI76_RS00730
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 146,062 + BWI76_RS00715 0.39 +0.2 146,068 + BWI76_RS00715 0.39 -2.2 146,146 - -1.0 146,232 - BWI76_RS00715 0.51 -1.4 146,232 - BWI76_RS00715 0.51 -1.4 146,345 - BWI76_RS00715 0.59 -1.3 146,348 + BWI76_RS00715 0.60 -0.9 146,554 + BWI76_RS00715 0.75 -0.0 147,037 + BWI76_RS00720 0.20 -2.3 147,129 + BWI76_RS00720 0.33 -1.7 147,356 - BWI76_RS00720 0.65 +1.6 147,524 - BWI76_RS00720 0.89 -0.4 147,661 + +0.7 147,669 - +1.0 147,669 - -3.1 147,861 - BWI76_RS00725 0.23 -0.3 147,897 - BWI76_RS00725 0.30 -0.4 148,215 + +1.3 148,368 + +0.4 148,411 - -0.8 148,416 - -0.0 148,431 - -0.3 148,453 + -0.6 148,500 - BWI76_RS00730 0.11 +0.5 148,503 - BWI76_RS00730 0.11 -0.3 148,575 - BWI76_RS00730 0.20 +1.4
Or see this region's nucleotide sequence