Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00705 and BWI76_RS00710 are separated by 71 nucleotides BWI76_RS00710 and BWI76_RS00715 are separated by 91 nucleotides BWI76_RS00715 and BWI76_RS00720 overlap by 4 nucleotides BWI76_RS00720 and BWI76_RS00725 are separated by 149 nucleotides
BWI76_RS00705: BWI76_RS00705 - superoxide dismutase, at 144,071 to 144,691
_RS00705
BWI76_RS00710: BWI76_RS00710 - 6-N-hydroxylaminopurine resistance protein, at 144,763 to 145,437
_RS00710
BWI76_RS00715: BWI76_RS00715 - two-component sensor histidine kinase, at 145,529 to 146,902
_RS00715
BWI76_RS00720: BWI76_RS00720 - DNA-binding response regulator, at 146,899 to 147,597
_RS00720
BWI76_RS00725: BWI76_RS00725 - periplasmic repressor CpxP, at 147,747 to 148,250
_RS00725
Position (kb) 145
146
147 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 144.704 kb on - strand at 144.802 kb on - strand at 145.079 kb on + strand, within BWI76_RS00710 at 145.087 kb on - strand, within BWI76_RS00710 at 145.478 kb on + strand at 145.560 kb on + strand at 145.686 kb on - strand, within BWI76_RS00715 at 146.062 kb on + strand, within BWI76_RS00715 at 146.068 kb on + strand, within BWI76_RS00715 at 146.146 kb on - strand at 146.232 kb on - strand, within BWI76_RS00715 at 146.232 kb on - strand, within BWI76_RS00715 at 146.345 kb on - strand, within BWI76_RS00715 at 146.348 kb on + strand, within BWI76_RS00715 at 146.554 kb on + strand, within BWI76_RS00715 at 147.037 kb on + strand, within BWI76_RS00720 at 147.129 kb on + strand, within BWI76_RS00720 at 147.356 kb on - strand, within BWI76_RS00720 at 147.524 kb on - strand, within BWI76_RS00720 at 147.661 kb on + strand at 147.669 kb on - strand at 147.669 kb on - strand at 147.861 kb on - strand, within BWI76_RS00725 at 147.897 kb on - strand, within BWI76_RS00725
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 144,704 - -0.5 144,802 - +0.1 145,079 + BWI76_RS00710 0.47 -0.9 145,087 - BWI76_RS00710 0.48 +0.3 145,478 + -0.5 145,560 + -0.8 145,686 - BWI76_RS00715 0.11 -1.0 146,062 + BWI76_RS00715 0.39 +0.2 146,068 + BWI76_RS00715 0.39 -2.2 146,146 - -1.0 146,232 - BWI76_RS00715 0.51 -1.4 146,232 - BWI76_RS00715 0.51 -1.4 146,345 - BWI76_RS00715 0.59 -1.3 146,348 + BWI76_RS00715 0.60 -0.9 146,554 + BWI76_RS00715 0.75 -0.0 147,037 + BWI76_RS00720 0.20 -2.3 147,129 + BWI76_RS00720 0.33 -1.7 147,356 - BWI76_RS00720 0.65 +1.6 147,524 - BWI76_RS00720 0.89 -0.4 147,661 + +0.7 147,669 - +1.0 147,669 - -3.1 147,861 - BWI76_RS00725 0.23 -0.3 147,897 - BWI76_RS00725 0.30 -0.4
Or see this region's nucleotide sequence