Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27230 and BWI76_RS27235 are separated by 9 nucleotides BWI76_RS27235 and BWI76_RS27240 are separated by 3 nucleotides BWI76_RS27240 and BWI76_RS27245 overlap by 4 nucleotides
BWI76_RS27230: BWI76_RS27230 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 5,650,762 to 5,652,306
_RS27230
BWI76_RS27235: BWI76_RS27235 - murein hydrolase activator EnvC, at 5,652,316 to 5,653,587
_RS27235
BWI76_RS27240: BWI76_RS27240 - hypothetical protein, at 5,653,591 to 5,654,523
_RS27240
BWI76_RS27245: BWI76_RS27245 - glycosyl transferase family protein, at 5,654,520 to 5,655,317
_RS27245
Position (kb)
5652
5653
5654 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5651.362 kb on + strand, within BWI76_RS27230 at 5651.400 kb on + strand, within BWI76_RS27230 at 5651.502 kb on - strand, within BWI76_RS27230 at 5651.664 kb on + strand, within BWI76_RS27230 at 5651.672 kb on - strand, within BWI76_RS27230 at 5651.966 kb on + strand, within BWI76_RS27230 at 5652.030 kb on - strand, within BWI76_RS27230 at 5652.104 kb on - strand, within BWI76_RS27230 at 5652.135 kb on + strand, within BWI76_RS27230 at 5652.135 kb on + strand, within BWI76_RS27230 at 5652.143 kb on - strand, within BWI76_RS27230 at 5652.301 kb on + strand at 5652.320 kb on + strand at 5652.395 kb on - strand at 5652.406 kb on - strand at 5652.467 kb on - strand, within BWI76_RS27235 at 5652.528 kb on + strand, within BWI76_RS27235 at 5652.528 kb on + strand, within BWI76_RS27235 at 5652.536 kb on - strand, within BWI76_RS27235 at 5652.540 kb on + strand, within BWI76_RS27235 at 5652.540 kb on + strand, within BWI76_RS27235 at 5652.546 kb on + strand, within BWI76_RS27235 at 5652.782 kb on - strand, within BWI76_RS27235 at 5652.842 kb on + strand, within BWI76_RS27235 at 5652.929 kb on - strand, within BWI76_RS27235 at 5653.100 kb on + strand, within BWI76_RS27235 at 5653.122 kb on + strand, within BWI76_RS27235 at 5653.148 kb on + strand, within BWI76_RS27235 at 5653.204 kb on - strand, within BWI76_RS27235 at 5653.266 kb on + strand, within BWI76_RS27235 at 5653.272 kb on + strand, within BWI76_RS27235 at 5653.323 kb on + strand, within BWI76_RS27235 at 5653.454 kb on - strand, within BWI76_RS27235 at 5653.464 kb on + strand at 5653.507 kb on + strand at 5653.562 kb on + strand at 5653.570 kb on - strand at 5653.643 kb on - strand at 5653.763 kb on - strand, within BWI76_RS27240 at 5653.879 kb on + strand, within BWI76_RS27240 at 5653.900 kb on - strand, within BWI76_RS27240 at 5653.903 kb on - strand, within BWI76_RS27240 at 5653.948 kb on - strand, within BWI76_RS27240 at 5653.962 kb on + strand, within BWI76_RS27240 at 5653.965 kb on - strand, within BWI76_RS27240 at 5653.998 kb on - strand, within BWI76_RS27240 at 5654.067 kb on + strand, within BWI76_RS27240 at 5654.085 kb on + strand, within BWI76_RS27240 at 5654.088 kb on - strand, within BWI76_RS27240 at 5654.187 kb on + strand, within BWI76_RS27240 at 5654.195 kb on - strand, within BWI76_RS27240 at 5654.223 kb on + strand, within BWI76_RS27240 at 5654.254 kb on + strand, within BWI76_RS27240 at 5654.254 kb on + strand, within BWI76_RS27240 at 5654.278 kb on + strand, within BWI76_RS27240 at 5654.295 kb on - strand, within BWI76_RS27240 at 5654.332 kb on - strand, within BWI76_RS27240 at 5654.568 kb on + strand at 5654.571 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 5,651,362 + BWI76_RS27230 0.39 +0.7 5,651,400 + BWI76_RS27230 0.41 -0.7 5,651,502 - BWI76_RS27230 0.48 -0.4 5,651,664 + BWI76_RS27230 0.58 +0.3 5,651,672 - BWI76_RS27230 0.59 -0.5 5,651,966 + BWI76_RS27230 0.78 -1.5 5,652,030 - BWI76_RS27230 0.82 -0.0 5,652,104 - BWI76_RS27230 0.87 +3.0 5,652,135 + BWI76_RS27230 0.89 +0.3 5,652,135 + BWI76_RS27230 0.89 -0.2 5,652,143 - BWI76_RS27230 0.89 +0.5 5,652,301 + -2.2 5,652,320 + -1.2 5,652,395 - -1.2 5,652,406 - -1.1 5,652,467 - BWI76_RS27235 0.12 -1.5 5,652,528 + BWI76_RS27235 0.17 -0.1 5,652,528 + BWI76_RS27235 0.17 +0.1 5,652,536 - BWI76_RS27235 0.17 -0.7 5,652,540 + BWI76_RS27235 0.18 -0.8 5,652,540 + BWI76_RS27235 0.18 -0.7 5,652,546 + BWI76_RS27235 0.18 -1.0 5,652,782 - BWI76_RS27235 0.37 -0.1 5,652,842 + BWI76_RS27235 0.41 -0.6 5,652,929 - BWI76_RS27235 0.48 -1.7 5,653,100 + BWI76_RS27235 0.62 -1.5 5,653,122 + BWI76_RS27235 0.63 -1.3 5,653,148 + BWI76_RS27235 0.65 +1.7 5,653,204 - BWI76_RS27235 0.70 -1.3 5,653,266 + BWI76_RS27235 0.75 -0.4 5,653,272 + BWI76_RS27235 0.75 -1.4 5,653,323 + BWI76_RS27235 0.79 +0.2 5,653,454 - BWI76_RS27235 0.89 -0.7 5,653,464 + -0.4 5,653,507 + -0.5 5,653,562 + -0.3 5,653,570 - -0.5 5,653,643 - +0.3 5,653,763 - BWI76_RS27240 0.18 -1.8 5,653,879 + BWI76_RS27240 0.31 -0.2 5,653,900 - BWI76_RS27240 0.33 -1.1 5,653,903 - BWI76_RS27240 0.33 -2.3 5,653,948 - BWI76_RS27240 0.38 -1.7 5,653,962 + BWI76_RS27240 0.40 +1.2 5,653,965 - BWI76_RS27240 0.40 -0.8 5,653,998 - BWI76_RS27240 0.44 -3.4 5,654,067 + BWI76_RS27240 0.51 -0.3 5,654,085 + BWI76_RS27240 0.53 -0.0 5,654,088 - BWI76_RS27240 0.53 -0.9 5,654,187 + BWI76_RS27240 0.64 -1.8 5,654,195 - BWI76_RS27240 0.65 -0.6 5,654,223 + BWI76_RS27240 0.68 +0.1 5,654,254 + BWI76_RS27240 0.71 +0.0 5,654,254 + BWI76_RS27240 0.71 -0.6 5,654,278 + BWI76_RS27240 0.74 -2.2 5,654,295 - BWI76_RS27240 0.75 +0.5 5,654,332 - BWI76_RS27240 0.79 +0.4 5,654,568 + -0.9 5,654,571 - +0.3
Or see this region's nucleotide sequence