Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26145

Experiment: KLEB14 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26135 and BWI76_RS26140 are separated by 62 nucleotidesBWI76_RS26140 and BWI76_RS26145 overlap by 4 nucleotidesBWI76_RS26145 and BWI76_RS26150 are separated by 24 nucleotides BWI76_RS26135: BWI76_RS26135 - Intracellular growth attenuator protein igaA, at 5,399,028 to 5,401,157 _RS26135 BWI76_RS26140: BWI76_RS26140 - GMP/IMP nucleotidase, at 5,401,220 to 5,401,903 _RS26140 BWI76_RS26145: BWI76_RS26145 - heat-shock protein Hsp15, at 5,401,900 to 5,402,301 _RS26145 BWI76_RS26150: BWI76_RS26150 - molecular chaperone Hsp33, at 5,402,326 to 5,403,204 _RS26150 Position (kb) 5401 5402 5403Strain fitness (log2 ratio) -1 0 1 2at 5401.280 kb on + strandat 5401.350 kb on + strand, within BWI76_RS26140at 5401.432 kb on + strand, within BWI76_RS26140at 5401.438 kb on - strand, within BWI76_RS26140at 5401.440 kb on - strand, within BWI76_RS26140at 5401.492 kb on - strand, within BWI76_RS26140at 5401.636 kb on + strand, within BWI76_RS26140at 5401.850 kb on - strandat 5401.886 kb on + strandat 5402.012 kb on + strand, within BWI76_RS26145at 5402.020 kb on - strand, within BWI76_RS26145at 5402.020 kb on - strand, within BWI76_RS26145at 5402.111 kb on + strand, within BWI76_RS26145at 5402.477 kb on + strand, within BWI76_RS26150at 5402.696 kb on - strand, within BWI76_RS26150at 5402.770 kb on - strand, within BWI76_RS26150at 5402.838 kb on + strand, within BWI76_RS26150at 5402.892 kb on - strand, within BWI76_RS26150

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1
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5,401,280 + +0.2
5,401,350 + BWI76_RS26140 0.19 -0.1
5,401,432 + BWI76_RS26140 0.31 +1.0
5,401,438 - BWI76_RS26140 0.32 -0.9
5,401,440 - BWI76_RS26140 0.32 +0.4
5,401,492 - BWI76_RS26140 0.40 -0.4
5,401,636 + BWI76_RS26140 0.61 +2.7
5,401,850 - +2.1
5,401,886 + -0.5
5,402,012 + BWI76_RS26145 0.28 -0.6
5,402,020 - BWI76_RS26145 0.30 -1.5
5,402,020 - BWI76_RS26145 0.30 -0.6
5,402,111 + BWI76_RS26145 0.52 -0.9
5,402,477 + BWI76_RS26150 0.17 +1.6
5,402,696 - BWI76_RS26150 0.42 +0.7
5,402,770 - BWI76_RS26150 0.51 -1.6
5,402,838 + BWI76_RS26150 0.58 -1.6
5,402,892 - BWI76_RS26150 0.64 +0.7

Or see this region's nucleotide sequence