Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24050 and BWI76_RS24055 are separated by 379 nucleotides BWI76_RS24055 and BWI76_RS24060 are separated by 75 nucleotides BWI76_RS24060 and BWI76_RS24065 are separated by 94 nucleotides
BWI76_RS24050: BWI76_RS24050 - methionine adenosyltransferase, at 5,018,179 to 5,019,333
_RS24050
BWI76_RS24055: BWI76_RS24055 - MFS transporter, at 5,019,713 to 5,021,107
_RS24055
BWI76_RS24060: BWI76_RS24060 - SprT family protein, at 5,021,183 to 5,021,683
_RS24060
BWI76_RS24065: BWI76_RS24065 - deoxyribonuclease I, at 5,021,778 to 5,022,485
_RS24065
Position (kb) 5019
5020
5021
5022 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5019.413 kb on + strand at 5019.421 kb on - strand at 5019.484 kb on + strand at 5019.717 kb on - strand at 5019.835 kb on - strand at 5019.835 kb on - strand at 5019.888 kb on + strand, within BWI76_RS24055 at 5019.922 kb on + strand, within BWI76_RS24055 at 5020.376 kb on - strand, within BWI76_RS24055 at 5020.410 kb on + strand, within BWI76_RS24055 at 5020.674 kb on + strand, within BWI76_RS24055 at 5020.764 kb on - strand, within BWI76_RS24055 at 5020.807 kb on - strand, within BWI76_RS24055 at 5021.081 kb on - strand at 5021.275 kb on + strand, within BWI76_RS24060 at 5021.283 kb on - strand, within BWI76_RS24060 at 5021.769 kb on + strand at 5021.875 kb on - strand, within BWI76_RS24065
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 5,019,413 + -2.1 5,019,421 - -0.9 5,019,484 + +1.0 5,019,717 - -2.4 5,019,835 - -1.0 5,019,835 - -2.4 5,019,888 + BWI76_RS24055 0.13 -3.9 5,019,922 + BWI76_RS24055 0.15 -3.7 5,020,376 - BWI76_RS24055 0.48 -1.8 5,020,410 + BWI76_RS24055 0.50 -1.6 5,020,674 + BWI76_RS24055 0.69 -2.0 5,020,764 - BWI76_RS24055 0.75 -1.2 5,020,807 - BWI76_RS24055 0.78 -1.1 5,021,081 - +2.6 5,021,275 + BWI76_RS24060 0.18 +0.1 5,021,283 - BWI76_RS24060 0.20 -1.0 5,021,769 + +0.3 5,021,875 - BWI76_RS24065 0.14 -2.0
Or see this region's nucleotide sequence