Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23000

Experiment: KLEB14 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22980 and BWI76_RS22985 are separated by 211 nucleotidesBWI76_RS22985 and BWI76_RS22990 are separated by 47 nucleotidesBWI76_RS22990 and BWI76_RS22995 are separated by 44 nucleotidesBWI76_RS22995 and BWI76_RS23000 are separated by 100 nucleotidesBWI76_RS23000 and BWI76_RS23005 are separated by 74 nucleotidesBWI76_RS23005 and BWI76_RS23010 overlap by 7 nucleotides BWI76_RS22980: BWI76_RS22980 - murein transglycosylase A, at 4,797,115 to 4,798,212 _RS22980 BWI76_RS22985: BWI76_RS22985 - tRNA-Met, at 4,798,424 to 4,798,500 _RS22985 BWI76_RS22990: BWI76_RS22990 - tRNA-Met, at 4,798,548 to 4,798,624 _RS22990 BWI76_RS22995: BWI76_RS22995 - tRNA-Met, at 4,798,669 to 4,798,745 _RS22995 BWI76_RS23000: BWI76_RS23000 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 4,798,846 to 4,799,901 _RS23000 BWI76_RS23005: BWI76_RS23005 - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 4,799,976 to 4,800,521 _RS23005 BWI76_RS23010: BWI76_RS23010 - cobyric acid synthase CobQ, at 4,800,515 to 4,802,038 _RS23010 Position (kb) 4798 4799 4800Strain fitness (log2 ratio) -3 -2 -1 0 1at 4797.987 kb on - strand, within BWI76_RS22980at 4797.989 kb on + strand, within BWI76_RS22980at 4798.027 kb on - strand, within BWI76_RS22980at 4798.112 kb on + strandat 4798.245 kb on - strandat 4799.001 kb on + strand, within BWI76_RS23000at 4799.051 kb on - strand, within BWI76_RS23000at 4799.933 kb on - strandat 4800.011 kb on + strandat 4800.013 kb on - strandat 4800.126 kb on - strand, within BWI76_RS23005at 4800.368 kb on - strand, within BWI76_RS23005at 4800.650 kb on + strandat 4800.697 kb on + strand, within BWI76_RS23010at 4800.891 kb on + strand, within BWI76_RS23010

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1
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4,797,987 - BWI76_RS22980 0.79 -0.8
4,797,989 + BWI76_RS22980 0.80 -1.2
4,798,027 - BWI76_RS22980 0.83 +0.4
4,798,112 + +0.3
4,798,245 - -0.1
4,799,001 + BWI76_RS23000 0.15 -1.6
4,799,051 - BWI76_RS23000 0.19 -0.3
4,799,933 - -2.9
4,800,011 + -1.0
4,800,013 - +0.2
4,800,126 - BWI76_RS23005 0.27 -1.1
4,800,368 - BWI76_RS23005 0.72 -0.6
4,800,650 + -0.4
4,800,697 + BWI76_RS23010 0.12 -1.6
4,800,891 + BWI76_RS23010 0.25 -0.9

Or see this region's nucleotide sequence