Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22980 and BWI76_RS22985 are separated by 211 nucleotides BWI76_RS22985 and BWI76_RS22990 are separated by 47 nucleotides BWI76_RS22990 and BWI76_RS22995 are separated by 44 nucleotides BWI76_RS22995 and BWI76_RS23000 are separated by 100 nucleotides BWI76_RS23000 and BWI76_RS23005 are separated by 74 nucleotides BWI76_RS23005 and BWI76_RS23010 overlap by 7 nucleotides
BWI76_RS22980: BWI76_RS22980 - murein transglycosylase A, at 4,797,115 to 4,798,212
_RS22980
BWI76_RS22985: BWI76_RS22985 - tRNA-Met, at 4,798,424 to 4,798,500
_RS22985
BWI76_RS22990: BWI76_RS22990 - tRNA-Met, at 4,798,548 to 4,798,624
_RS22990
BWI76_RS22995: BWI76_RS22995 - tRNA-Met, at 4,798,669 to 4,798,745
_RS22995
BWI76_RS23000: BWI76_RS23000 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 4,798,846 to 4,799,901
_RS23000
BWI76_RS23005: BWI76_RS23005 - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 4,799,976 to 4,800,521
_RS23005
BWI76_RS23010: BWI76_RS23010 - cobyric acid synthase CobQ, at 4,800,515 to 4,802,038
_RS23010
Position (kb)
4798
4799
4800 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4797.987 kb on - strand, within BWI76_RS22980 at 4797.989 kb on + strand, within BWI76_RS22980 at 4798.027 kb on - strand, within BWI76_RS22980 at 4798.112 kb on + strand at 4798.245 kb on - strand at 4799.001 kb on + strand, within BWI76_RS23000 at 4799.051 kb on - strand, within BWI76_RS23000 at 4799.933 kb on - strand at 4800.011 kb on + strand at 4800.013 kb on - strand at 4800.126 kb on - strand, within BWI76_RS23005 at 4800.368 kb on - strand, within BWI76_RS23005 at 4800.650 kb on + strand at 4800.697 kb on + strand, within BWI76_RS23010 at 4800.891 kb on + strand, within BWI76_RS23010
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 4,797,987 - BWI76_RS22980 0.79 -0.8 4,797,989 + BWI76_RS22980 0.80 -1.2 4,798,027 - BWI76_RS22980 0.83 +0.4 4,798,112 + +0.3 4,798,245 - -0.1 4,799,001 + BWI76_RS23000 0.15 -1.6 4,799,051 - BWI76_RS23000 0.19 -0.3 4,799,933 - -2.9 4,800,011 + -1.0 4,800,013 - +0.2 4,800,126 - BWI76_RS23005 0.27 -1.1 4,800,368 - BWI76_RS23005 0.72 -0.6 4,800,650 + -0.4 4,800,697 + BWI76_RS23010 0.12 -1.6 4,800,891 + BWI76_RS23010 0.25 -0.9
Or see this region's nucleotide sequence