Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21030 and BWI76_RS21035 are separated by 158 nucleotides BWI76_RS21035 and BWI76_RS21040 are separated by 81 nucleotides
BWI76_RS21030: BWI76_RS21030 - IMP dehydrogenase, at 4,400,678 to 4,402,144
_RS21030
BWI76_RS21035: BWI76_RS21035 - exodeoxyribonuclease VII large subunit, at 4,402,303 to 4,403,676
_RS21035
BWI76_RS21040: BWI76_RS21040 - peptidase M4, at 4,403,758 to 4,404,786
_RS21040
Position (kb)
4402
4403
4404 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4402.183 kb on + strand at 4402.191 kb on - strand at 4402.243 kb on + strand at 4402.243 kb on + strand at 4402.253 kb on + strand at 4402.253 kb on + strand at 4402.548 kb on - strand, within BWI76_RS21035 at 4402.548 kb on - strand, within BWI76_RS21035 at 4402.660 kb on + strand, within BWI76_RS21035 at 4402.732 kb on - strand, within BWI76_RS21035 at 4402.833 kb on + strand, within BWI76_RS21035 at 4402.833 kb on + strand, within BWI76_RS21035 at 4402.833 kb on + strand, within BWI76_RS21035 at 4402.841 kb on - strand, within BWI76_RS21035 at 4402.841 kb on - strand, within BWI76_RS21035 at 4402.865 kb on + strand, within BWI76_RS21035 at 4402.993 kb on - strand, within BWI76_RS21035 at 4403.434 kb on + strand, within BWI76_RS21035 at 4403.830 kb on - strand at 4403.858 kb on - strand at 4403.978 kb on + strand, within BWI76_RS21040 at 4404.006 kb on - strand, within BWI76_RS21040 at 4404.042 kb on - strand, within BWI76_RS21040 at 4404.267 kb on + strand, within BWI76_RS21040 at 4404.390 kb on - strand, within BWI76_RS21040 at 4404.450 kb on + strand, within BWI76_RS21040
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 4,402,183 + -1.0 4,402,191 - -0.8 4,402,243 + +0.8 4,402,243 + +0.3 4,402,253 + +0.0 4,402,253 + -1.0 4,402,548 - BWI76_RS21035 0.18 -1.1 4,402,548 - BWI76_RS21035 0.18 +3.0 4,402,660 + BWI76_RS21035 0.26 -0.5 4,402,732 - BWI76_RS21035 0.31 -0.7 4,402,833 + BWI76_RS21035 0.39 -1.1 4,402,833 + BWI76_RS21035 0.39 -1.2 4,402,833 + BWI76_RS21035 0.39 -0.2 4,402,841 - BWI76_RS21035 0.39 -1.3 4,402,841 - BWI76_RS21035 0.39 -0.7 4,402,865 + BWI76_RS21035 0.41 +0.3 4,402,993 - BWI76_RS21035 0.50 -1.4 4,403,434 + BWI76_RS21035 0.82 -0.3 4,403,830 - -1.2 4,403,858 - -1.8 4,403,978 + BWI76_RS21040 0.21 +0.2 4,404,006 - BWI76_RS21040 0.24 +0.0 4,404,042 - BWI76_RS21040 0.28 +0.2 4,404,267 + BWI76_RS21040 0.49 +0.6 4,404,390 - BWI76_RS21040 0.61 +0.1 4,404,450 + BWI76_RS21040 0.67 -2.6
Or see this region's nucleotide sequence