Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19140 and BWI76_RS19145 are separated by 280 nucleotides BWI76_RS19145 and BWI76_RS19150 overlap by 4 nucleotides BWI76_RS19150 and BWI76_RS19155 are separated by 12 nucleotides
BWI76_RS19140: BWI76_RS19140 - citrate (Si)-synthase, at 3,979,437 to 3,980,723
_RS19140
BWI76_RS19145: BWI76_RS19145 - formate dehydrogenase cytochrome b556 subunit, at 3,981,004 to 3,981,633
_RS19145
BWI76_RS19150: BWI76_RS19150 - formate dehydrogenase subunit beta, at 3,981,630 to 3,982,532
_RS19150
BWI76_RS19155: BWI76_RS19155 - formate dehydrogenase-N subunit alpha, at 3,982,545 to 3,985,595
_RS19155
Position (kb)
3981
3982 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3980.306 kb on + strand, within BWI76_RS19140 at 3980.319 kb on + strand, within BWI76_RS19140 at 3980.319 kb on + strand, within BWI76_RS19140 at 3980.327 kb on - strand, within BWI76_RS19140 at 3980.484 kb on - strand, within BWI76_RS19140 at 3980.662 kb on + strand at 3981.070 kb on - strand, within BWI76_RS19145
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 3,980,306 + BWI76_RS19140 0.68 -0.5 3,980,319 + BWI76_RS19140 0.69 +1.9 3,980,319 + BWI76_RS19140 0.69 +3.5 3,980,327 - BWI76_RS19140 0.69 -1.6 3,980,484 - BWI76_RS19140 0.81 -0.2 3,980,662 + -0.7 3,981,070 - BWI76_RS19145 0.10 -1.6
Or see this region's nucleotide sequence