Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16970 and BWI76_RS16975 are separated by 101 nucleotides BWI76_RS16975 and BWI76_RS16980 are separated by 11 nucleotides BWI76_RS16980 and BWI76_RS16985 overlap by 4 nucleotides
BWI76_RS16970: BWI76_RS16970 - murein L,D-transpeptidase, at 3,519,587 to 3,520,570
_RS16970
BWI76_RS16975: BWI76_RS16975 - cysteine desufuration protein SufE, at 3,520,672 to 3,521,088
_RS16975
BWI76_RS16980: BWI76_RS16980 - bifunctional cysteine desulfurase/selenocysteine lyase, at 3,521,100 to 3,522,320
_RS16980
BWI76_RS16985: BWI76_RS16985 - signal peptide peptidase SppA, at 3,522,317 to 3,523,591
_RS16985
Position (kb)
3521
3522
3523 Strain fitness (log2 ratio)
-2
-1
0
1 at 3520.206 kb on + strand, within BWI76_RS16970 at 3520.272 kb on - strand, within BWI76_RS16970 at 3520.441 kb on - strand, within BWI76_RS16970 at 3520.443 kb on + strand, within BWI76_RS16970 at 3520.550 kb on + strand at 3520.648 kb on - strand at 3520.953 kb on + strand, within BWI76_RS16975 at 3521.043 kb on + strand, within BWI76_RS16975 at 3521.051 kb on - strand at 3521.158 kb on + strand at 3521.161 kb on + strand at 3521.169 kb on - strand at 3521.211 kb on - strand at 3521.367 kb on + strand, within BWI76_RS16980 at 3521.390 kb on + strand, within BWI76_RS16980 at 3521.708 kb on - strand, within BWI76_RS16980 at 3522.043 kb on - strand, within BWI76_RS16980 at 3522.327 kb on + strand at 3522.647 kb on + strand, within BWI76_RS16985
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 3,520,206 + BWI76_RS16970 0.63 -0.8 3,520,272 - BWI76_RS16970 0.70 -0.1 3,520,441 - BWI76_RS16970 0.87 +0.0 3,520,443 + BWI76_RS16970 0.87 +0.1 3,520,550 + +0.5 3,520,648 - -1.0 3,520,953 + BWI76_RS16975 0.67 +1.1 3,521,043 + BWI76_RS16975 0.89 -0.2 3,521,051 - +0.8 3,521,158 + -0.2 3,521,161 + -0.5 3,521,169 - +0.8 3,521,211 - +0.3 3,521,367 + BWI76_RS16980 0.22 -2.5 3,521,390 + BWI76_RS16980 0.24 -0.7 3,521,708 - BWI76_RS16980 0.50 -0.5 3,522,043 - BWI76_RS16980 0.77 -0.6 3,522,327 + -0.7 3,522,647 + BWI76_RS16985 0.26 -0.2
Or see this region's nucleotide sequence