Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15935 and BWI76_RS15940 are separated by 41 nucleotides BWI76_RS15940 and BWI76_RS15945 are separated by 139 nucleotides BWI76_RS15945 and BWI76_RS15950 overlap by 48 nucleotides BWI76_RS15950 and BWI76_RS15955 are separated by 127 nucleotides
BWI76_RS15935: BWI76_RS15935 - dimethyl sulfoxide reductase, at 3,305,324 to 3,306,181
_RS15935
BWI76_RS15940: BWI76_RS15940 - DMSO reductase maturation protein DsmD, at 3,306,223 to 3,306,831
_RS15940
BWI76_RS15945: BWI76_RS15945 - voltage-gated ClC-type chloride channel ClcB, at 3,306,971 to 3,308,266
_RS15945
BWI76_RS15950: BWI76_RS15950 - dethiobiotin synthase, at 3,308,219 to 3,308,914
_RS15950
BWI76_RS15955: BWI76_RS15955 - transcriptional regulator, at 3,309,042 to 3,310,262
_RS15955
Position (kb)
3306
3307
3308
3309 Strain fitness (log2 ratio)
-1
0
1
2 at 3306.388 kb on + strand, within BWI76_RS15940 at 3306.753 kb on - strand, within BWI76_RS15940 at 3306.795 kb on + strand at 3306.809 kb on + strand at 3306.832 kb on + strand at 3307.119 kb on + strand, within BWI76_RS15945 at 3307.760 kb on - strand, within BWI76_RS15945 at 3308.117 kb on - strand, within BWI76_RS15945 at 3308.662 kb on - strand, within BWI76_RS15950 at 3308.806 kb on + strand, within BWI76_RS15950 at 3308.846 kb on - strand at 3309.001 kb on + strand at 3309.088 kb on + strand at 3309.088 kb on + strand at 3309.093 kb on + strand at 3309.101 kb on - strand at 3309.235 kb on - strand, within BWI76_RS15955 at 3309.236 kb on - strand, within BWI76_RS15955
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 3,306,388 + BWI76_RS15940 0.27 +2.1 3,306,753 - BWI76_RS15940 0.87 +0.3 3,306,795 + +0.2 3,306,809 + +0.0 3,306,832 + +0.3 3,307,119 + BWI76_RS15945 0.11 -0.7 3,307,760 - BWI76_RS15945 0.61 -1.2 3,308,117 - BWI76_RS15945 0.88 -0.6 3,308,662 - BWI76_RS15950 0.64 -1.2 3,308,806 + BWI76_RS15950 0.84 +1.7 3,308,846 - +1.0 3,309,001 + +1.5 3,309,088 + +0.3 3,309,088 + +1.5 3,309,093 + +0.1 3,309,101 - +1.7 3,309,235 - BWI76_RS15955 0.16 +0.9 3,309,236 - BWI76_RS15955 0.16 -1.6
Or see this region's nucleotide sequence