Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15945

Experiment: KLEB14 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15935 and BWI76_RS15940 are separated by 41 nucleotidesBWI76_RS15940 and BWI76_RS15945 are separated by 139 nucleotidesBWI76_RS15945 and BWI76_RS15950 overlap by 48 nucleotidesBWI76_RS15950 and BWI76_RS15955 are separated by 127 nucleotides BWI76_RS15935: BWI76_RS15935 - dimethyl sulfoxide reductase, at 3,305,324 to 3,306,181 _RS15935 BWI76_RS15940: BWI76_RS15940 - DMSO reductase maturation protein DsmD, at 3,306,223 to 3,306,831 _RS15940 BWI76_RS15945: BWI76_RS15945 - voltage-gated ClC-type chloride channel ClcB, at 3,306,971 to 3,308,266 _RS15945 BWI76_RS15950: BWI76_RS15950 - dethiobiotin synthase, at 3,308,219 to 3,308,914 _RS15950 BWI76_RS15955: BWI76_RS15955 - transcriptional regulator, at 3,309,042 to 3,310,262 _RS15955 Position (kb) 3306 3307 3308 3309Strain fitness (log2 ratio) -1 0 1 2at 3306.388 kb on + strand, within BWI76_RS15940at 3306.753 kb on - strand, within BWI76_RS15940at 3306.795 kb on + strandat 3306.809 kb on + strandat 3306.832 kb on + strandat 3307.119 kb on + strand, within BWI76_RS15945at 3307.760 kb on - strand, within BWI76_RS15945at 3308.117 kb on - strand, within BWI76_RS15945at 3308.662 kb on - strand, within BWI76_RS15950at 3308.806 kb on + strand, within BWI76_RS15950at 3308.846 kb on - strandat 3309.001 kb on + strandat 3309.088 kb on + strandat 3309.088 kb on + strandat 3309.093 kb on + strandat 3309.101 kb on - strandat 3309.235 kb on - strand, within BWI76_RS15955at 3309.236 kb on - strand, within BWI76_RS15955

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1
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3,306,388 + BWI76_RS15940 0.27 +2.1
3,306,753 - BWI76_RS15940 0.87 +0.3
3,306,795 + +0.2
3,306,809 + +0.0
3,306,832 + +0.3
3,307,119 + BWI76_RS15945 0.11 -0.7
3,307,760 - BWI76_RS15945 0.61 -1.2
3,308,117 - BWI76_RS15945 0.88 -0.6
3,308,662 - BWI76_RS15950 0.64 -1.2
3,308,806 + BWI76_RS15950 0.84 +1.7
3,308,846 - +1.0
3,309,001 + +1.5
3,309,088 + +0.3
3,309,088 + +1.5
3,309,093 + +0.1
3,309,101 - +1.7
3,309,235 - BWI76_RS15955 0.16 +0.9
3,309,236 - BWI76_RS15955 0.16 -1.6

Or see this region's nucleotide sequence