Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12705 and BWI76_RS12710 are separated by 43 nucleotides BWI76_RS12710 and BWI76_RS12715 are separated by 193 nucleotides BWI76_RS12715 and BWI76_RS12720 are separated by 82 nucleotides
BWI76_RS12705: BWI76_RS12705 - ATP-dependent RNA helicase, at 2,648,224 to 2,649,597
_RS12705
BWI76_RS12710: BWI76_RS12710 - tRNA 2-thiocytidine(32) synthetase TtcA, at 2,649,641 to 2,650,576
_RS12710
BWI76_RS12715: BWI76_RS12715 - universal stress protein F, at 2,650,770 to 2,651,204
_RS12715
BWI76_RS12720: BWI76_RS12720 - KTSC domain-containing protein, at 2,651,287 to 2,651,499
_RS12720
Position (kb)
2649
2650
2651 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2648.662 kb on + strand, within BWI76_RS12705 at 2648.673 kb on + strand, within BWI76_RS12705 at 2648.815 kb on + strand, within BWI76_RS12705 at 2649.044 kb on + strand, within BWI76_RS12705 at 2649.044 kb on + strand, within BWI76_RS12705 at 2649.533 kb on + strand at 2649.618 kb on - strand at 2649.618 kb on - strand at 2649.766 kb on + strand, within BWI76_RS12710 at 2649.825 kb on - strand, within BWI76_RS12710 at 2649.843 kb on + strand, within BWI76_RS12710 at 2649.878 kb on - strand, within BWI76_RS12710 at 2649.944 kb on - strand, within BWI76_RS12710 at 2650.099 kb on + strand, within BWI76_RS12710 at 2650.159 kb on + strand, within BWI76_RS12710 at 2650.198 kb on - strand, within BWI76_RS12710 at 2650.426 kb on + strand, within BWI76_RS12710 at 2650.673 kb on - strand at 2650.765 kb on - strand at 2650.843 kb on - strand, within BWI76_RS12715 at 2651.016 kb on - strand, within BWI76_RS12715 at 2651.016 kb on - strand, within BWI76_RS12715 at 2651.041 kb on + strand, within BWI76_RS12715 at 2651.041 kb on + strand, within BWI76_RS12715 at 2651.041 kb on + strand, within BWI76_RS12715 at 2651.049 kb on - strand, within BWI76_RS12715 at 2651.062 kb on + strand, within BWI76_RS12715 at 2651.388 kb on + strand, within BWI76_RS12720 at 2651.390 kb on + strand, within BWI76_RS12720 at 2651.390 kb on + strand, within BWI76_RS12720 at 2651.398 kb on - strand, within BWI76_RS12720 at 2651.398 kb on - strand, within BWI76_RS12720 at 2651.403 kb on + strand, within BWI76_RS12720 at 2651.405 kb on - strand, within BWI76_RS12720 at 2651.445 kb on + strand, within BWI76_RS12720 at 2651.480 kb on + strand at 2651.488 kb on - strand at 2651.494 kb on + strand at 2651.504 kb on + strand at 2651.527 kb on + strand at 2651.535 kb on - strand at 2651.545 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 2,648,662 + BWI76_RS12705 0.32 -0.7 2,648,673 + BWI76_RS12705 0.33 -0.5 2,648,815 + BWI76_RS12705 0.43 +0.6 2,649,044 + BWI76_RS12705 0.60 +0.5 2,649,044 + BWI76_RS12705 0.60 +0.1 2,649,533 + -0.1 2,649,618 - +0.4 2,649,618 - +1.5 2,649,766 + BWI76_RS12710 0.13 +1.9 2,649,825 - BWI76_RS12710 0.20 +1.0 2,649,843 + BWI76_RS12710 0.22 +1.5 2,649,878 - BWI76_RS12710 0.25 +0.9 2,649,944 - BWI76_RS12710 0.32 +0.2 2,650,099 + BWI76_RS12710 0.49 +1.7 2,650,159 + BWI76_RS12710 0.55 -0.7 2,650,198 - BWI76_RS12710 0.60 +0.3 2,650,426 + BWI76_RS12710 0.84 +0.5 2,650,673 - -2.1 2,650,765 - +0.4 2,650,843 - BWI76_RS12715 0.17 -0.3 2,651,016 - BWI76_RS12715 0.57 +2.7 2,651,016 - BWI76_RS12715 0.57 -0.9 2,651,041 + BWI76_RS12715 0.62 +0.7 2,651,041 + BWI76_RS12715 0.62 +1.1 2,651,041 + BWI76_RS12715 0.62 +0.9 2,651,049 - BWI76_RS12715 0.64 -0.5 2,651,062 + BWI76_RS12715 0.67 +0.3 2,651,388 + BWI76_RS12720 0.47 +1.2 2,651,390 + BWI76_RS12720 0.48 -1.2 2,651,390 + BWI76_RS12720 0.48 +1.2 2,651,398 - BWI76_RS12720 0.52 -0.4 2,651,398 - BWI76_RS12720 0.52 -0.8 2,651,403 + BWI76_RS12720 0.54 +2.9 2,651,405 - BWI76_RS12720 0.55 +0.7 2,651,445 + BWI76_RS12720 0.74 +0.7 2,651,480 + +1.3 2,651,488 - +0.1 2,651,494 + -1.5 2,651,504 + -0.1 2,651,527 + -0.8 2,651,535 - -0.1 2,651,545 - -0.6
Or see this region's nucleotide sequence