Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10565 and BWI76_RS10570 are separated by 214 nucleotides BWI76_RS10570 and BWI76_RS10575 are separated by 9 nucleotides BWI76_RS10575 and BWI76_RS10580 overlap by 1 nucleotides
BWI76_RS10565: BWI76_RS10565 - sugar isomerase, at 2,231,816 to 2,232,655
_RS10565
BWI76_RS10570: BWI76_RS10570 - cysteine ABC transporter substrate-binding protein, at 2,232,870 to 2,233,655
_RS10570
BWI76_RS10575: BWI76_RS10575 - polar amino acid ABC transporter permease, at 2,233,665 to 2,234,330
_RS10575
BWI76_RS10580: BWI76_RS10580 - amino acid ABC transporter permease, at 2,234,330 to 2,234,986
_RS10580
Position (kb)
2232
2233
2234 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2231.926 kb on - strand, within BWI76_RS10565 at 2232.085 kb on - strand, within BWI76_RS10565 at 2232.153 kb on + strand, within BWI76_RS10565 at 2232.155 kb on - strand, within BWI76_RS10565 at 2232.228 kb on - strand, within BWI76_RS10565 at 2232.320 kb on + strand, within BWI76_RS10565 at 2232.893 kb on + strand at 2232.893 kb on + strand at 2233.032 kb on - strand, within BWI76_RS10570 at 2233.157 kb on - strand, within BWI76_RS10570 at 2233.221 kb on - strand, within BWI76_RS10570 at 2233.384 kb on - strand, within BWI76_RS10570 at 2233.713 kb on - strand at 2233.851 kb on - strand, within BWI76_RS10575 at 2233.978 kb on + strand, within BWI76_RS10575 at 2234.275 kb on - strand at 2234.506 kb on + strand, within BWI76_RS10580 at 2234.514 kb on - strand, within BWI76_RS10580
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 2,231,926 - BWI76_RS10565 0.13 +1.2 2,232,085 - BWI76_RS10565 0.32 +0.1 2,232,153 + BWI76_RS10565 0.40 +1.1 2,232,155 - BWI76_RS10565 0.40 -1.4 2,232,228 - BWI76_RS10565 0.49 -0.1 2,232,320 + BWI76_RS10565 0.60 -0.1 2,232,893 + -2.3 2,232,893 + +2.7 2,233,032 - BWI76_RS10570 0.21 -1.0 2,233,157 - BWI76_RS10570 0.37 -1.6 2,233,221 - BWI76_RS10570 0.45 -0.4 2,233,384 - BWI76_RS10570 0.65 -0.6 2,233,713 - -0.5 2,233,851 - BWI76_RS10575 0.28 -1.1 2,233,978 + BWI76_RS10575 0.47 +0.4 2,234,275 - +1.7 2,234,506 + BWI76_RS10580 0.27 +0.7 2,234,514 - BWI76_RS10580 0.28 -0.9
Or see this region's nucleotide sequence