Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09475 and BWI76_RS09480 are separated by 57 nucleotides BWI76_RS09480 and BWI76_RS09485 are separated by 42 nucleotides BWI76_RS09485 and BWI76_RS09490 are separated by 78 nucleotides BWI76_RS09490 and BWI76_RS09495 overlap by 4 nucleotides
BWI76_RS09475: BWI76_RS09475 - putrescine ABC transporter permease PotI, at 1,983,885 to 1,984,730
_RS09475
BWI76_RS09480: BWI76_RS09480 - hypothetical protein, at 1,984,788 to 1,985,276
_RS09480
BWI76_RS09485: BWI76_RS09485 - 23S rRNA (uracil(747)-C(5))-methyltransferase, at 1,985,319 to 1,986,449
_RS09485
BWI76_RS09490: BWI76_RS09490 - DNA-binding response regulator, at 1,986,528 to 1,987,244
_RS09490
BWI76_RS09495: BWI76_RS09495 - sensor histidine kinase, at 1,987,241 to 1,988,713
_RS09495
Position (kb) 1985
1986
1987 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 1984.331 kb on + strand, within BWI76_RS09475 at 1984.339 kb on - strand, within BWI76_RS09475 at 1984.378 kb on + strand, within BWI76_RS09475 at 1984.495 kb on + strand, within BWI76_RS09475 at 1984.503 kb on - strand, within BWI76_RS09475 at 1984.710 kb on - strand at 1984.777 kb on - strand at 1984.847 kb on - strand, within BWI76_RS09480 at 1984.859 kb on + strand, within BWI76_RS09480 at 1984.973 kb on - strand, within BWI76_RS09480 at 1984.973 kb on - strand, within BWI76_RS09480 at 1985.132 kb on + strand, within BWI76_RS09480 at 1985.196 kb on - strand, within BWI76_RS09480 at 1985.279 kb on + strand at 1985.455 kb on - strand, within BWI76_RS09485 at 1985.681 kb on + strand, within BWI76_RS09485 at 1985.690 kb on - strand, within BWI76_RS09485 at 1985.776 kb on - strand, within BWI76_RS09485 at 1986.046 kb on - strand, within BWI76_RS09485 at 1986.063 kb on - strand, within BWI76_RS09485 at 1986.350 kb on - strand at 1986.420 kb on + strand at 1986.548 kb on + strand at 1986.970 kb on + strand, within BWI76_RS09490 at 1986.970 kb on + strand, within BWI76_RS09490 at 1987.433 kb on - strand, within BWI76_RS09495
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 1,984,331 + BWI76_RS09475 0.53 +0.6 1,984,339 - BWI76_RS09475 0.54 -0.5 1,984,378 + BWI76_RS09475 0.58 +1.5 1,984,495 + BWI76_RS09475 0.72 +1.1 1,984,503 - BWI76_RS09475 0.73 +0.2 1,984,710 - -0.7 1,984,777 - +0.3 1,984,847 - BWI76_RS09480 0.12 -0.1 1,984,859 + BWI76_RS09480 0.15 +0.0 1,984,973 - BWI76_RS09480 0.38 +0.3 1,984,973 - BWI76_RS09480 0.38 +0.1 1,985,132 + BWI76_RS09480 0.70 +0.3 1,985,196 - BWI76_RS09480 0.83 +0.8 1,985,279 + -0.5 1,985,455 - BWI76_RS09485 0.12 -0.1 1,985,681 + BWI76_RS09485 0.32 +1.1 1,985,690 - BWI76_RS09485 0.33 +1.6 1,985,776 - BWI76_RS09485 0.40 +0.9 1,986,046 - BWI76_RS09485 0.64 +0.0 1,986,063 - BWI76_RS09485 0.66 +1.2 1,986,350 - -0.2 1,986,420 + -0.1 1,986,548 + -0.7 1,986,970 + BWI76_RS09490 0.62 +0.1 1,986,970 + BWI76_RS09490 0.62 -0.6 1,987,433 - BWI76_RS09495 0.13 +4.8
Or see this region's nucleotide sequence