Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07065 and BWI76_RS07070 overlap by 4 nucleotides BWI76_RS07070 and BWI76_RS07075 are separated by 96 nucleotides BWI76_RS07075 and BWI76_RS07080 are separated by 106 nucleotides BWI76_RS07080 and BWI76_RS07085 are separated by 3 nucleotides
BWI76_RS07065: BWI76_RS07065 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 1,486,550 to 1,487,617
_RS07065
BWI76_RS07070: BWI76_RS07070 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 1,487,614 to 1,488,123
_RS07070
BWI76_RS07075: BWI76_RS07075 - hypothetical protein, at 1,488,220 to 1,488,549
_RS07075
BWI76_RS07080: BWI76_RS07080 - UDP-2,3-diacylglucosamine diphosphatase, at 1,488,656 to 1,489,378
_RS07080
BWI76_RS07085: BWI76_RS07085 - peptidyl-prolyl cis-trans isomerase, at 1,489,382 to 1,489,876
_RS07085
Position (kb)
1488
1489 Strain fitness (log2 ratio)
-1
0
1
2 at 1487.222 kb on + strand, within BWI76_RS07065 at 1487.293 kb on - strand, within BWI76_RS07065 at 1487.293 kb on - strand, within BWI76_RS07065 at 1487.639 kb on - strand at 1487.641 kb on + strand at 1487.694 kb on + strand, within BWI76_RS07070 at 1487.835 kb on - strand, within BWI76_RS07070 at 1487.847 kb on - strand, within BWI76_RS07070 at 1487.901 kb on - strand, within BWI76_RS07070 at 1487.962 kb on - strand, within BWI76_RS07070 at 1488.071 kb on + strand, within BWI76_RS07070 at 1488.148 kb on + strand at 1488.156 kb on - strand at 1488.158 kb on + strand at 1488.158 kb on + strand at 1488.344 kb on + strand, within BWI76_RS07075 at 1488.344 kb on + strand, within BWI76_RS07075 at 1488.375 kb on + strand, within BWI76_RS07075 at 1488.435 kb on + strand, within BWI76_RS07075 at 1489.529 kb on - strand, within BWI76_RS07085
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 1,487,222 + BWI76_RS07065 0.63 -1.1 1,487,293 - BWI76_RS07065 0.70 +0.1 1,487,293 - BWI76_RS07065 0.70 +1.2 1,487,639 - +0.3 1,487,641 + +2.2 1,487,694 + BWI76_RS07070 0.16 +0.3 1,487,835 - BWI76_RS07070 0.43 -1.1 1,487,847 - BWI76_RS07070 0.46 +1.0 1,487,901 - BWI76_RS07070 0.56 +0.8 1,487,962 - BWI76_RS07070 0.68 -1.0 1,488,071 + BWI76_RS07070 0.90 +0.5 1,488,148 + -0.4 1,488,156 - -1.6 1,488,158 + +0.9 1,488,158 + +1.2 1,488,344 + BWI76_RS07075 0.38 -0.6 1,488,344 + BWI76_RS07075 0.38 -0.7 1,488,375 + BWI76_RS07075 0.47 +2.3 1,488,435 + BWI76_RS07075 0.65 -0.1 1,489,529 - BWI76_RS07085 0.30 -1.7
Or see this region's nucleotide sequence