Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05340 and BWI76_RS05345 are separated by 110 nucleotides BWI76_RS05345 and BWI76_RS05350 overlap by 4 nucleotides BWI76_RS05350 and BWI76_RS05355 are separated by 108 nucleotides BWI76_RS05355 and BWI76_RS05360 are separated by 39 nucleotides
BWI76_RS05340: BWI76_RS05340 - proline--tRNA ligase, at 1,124,100 to 1,125,818
_RS05340
BWI76_RS05345: BWI76_RS05345 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,125,929 to 1,126,636
_RS05345
BWI76_RS05350: BWI76_RS05350 - Rcs stress response system protein RcsF, at 1,126,633 to 1,127,040
_RS05350
BWI76_RS05355: BWI76_RS05355 - methionine ABC transporter substrate-binding protein MetQ, at 1,127,149 to 1,127,964
_RS05355
BWI76_RS05360: BWI76_RS05360 - methionine ABC transporter permease, at 1,128,004 to 1,128,657
_RS05360
Position (kb)
1126
1127
1128 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1126.008 kb on + strand, within BWI76_RS05345 at 1126.016 kb on - strand, within BWI76_RS05345 at 1126.016 kb on - strand, within BWI76_RS05345 at 1126.016 kb on - strand, within BWI76_RS05345 at 1126.098 kb on - strand, within BWI76_RS05345 at 1126.227 kb on + strand, within BWI76_RS05345 at 1126.434 kb on + strand, within BWI76_RS05345 at 1126.487 kb on + strand, within BWI76_RS05345 at 1126.507 kb on + strand, within BWI76_RS05345 at 1126.515 kb on - strand, within BWI76_RS05345 at 1126.553 kb on - strand, within BWI76_RS05345 at 1126.555 kb on + strand, within BWI76_RS05345 at 1126.659 kb on + strand at 1126.677 kb on + strand, within BWI76_RS05350 at 1126.695 kb on - strand, within BWI76_RS05350 at 1126.751 kb on + strand, within BWI76_RS05350 at 1126.759 kb on - strand, within BWI76_RS05350 at 1126.954 kb on + strand, within BWI76_RS05350 at 1126.962 kb on - strand, within BWI76_RS05350 at 1126.979 kb on + strand, within BWI76_RS05350 at 1126.986 kb on + strand, within BWI76_RS05350 at 1126.986 kb on + strand, within BWI76_RS05350 at 1126.986 kb on + strand, within BWI76_RS05350 at 1126.989 kb on - strand, within BWI76_RS05350 at 1127.008 kb on - strand at 1127.138 kb on - strand at 1127.155 kb on + strand at 1127.163 kb on - strand at 1127.214 kb on + strand at 1127.221 kb on - strand at 1127.301 kb on - strand, within BWI76_RS05355 at 1127.301 kb on - strand, within BWI76_RS05355 at 1127.302 kb on - strand, within BWI76_RS05355 at 1127.542 kb on + strand, within BWI76_RS05355 at 1127.690 kb on + strand, within BWI76_RS05355 at 1127.693 kb on - strand, within BWI76_RS05355 at 1127.698 kb on - strand, within BWI76_RS05355 at 1127.698 kb on - strand, within BWI76_RS05355 at 1127.892 kb on + strand at 1127.945 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 1,126,008 + BWI76_RS05345 0.11 +0.4 1,126,016 - BWI76_RS05345 0.12 +0.6 1,126,016 - BWI76_RS05345 0.12 -0.6 1,126,016 - BWI76_RS05345 0.12 -0.5 1,126,098 - BWI76_RS05345 0.24 -1.1 1,126,227 + BWI76_RS05345 0.42 +2.5 1,126,434 + BWI76_RS05345 0.71 +0.7 1,126,487 + BWI76_RS05345 0.79 -0.1 1,126,507 + BWI76_RS05345 0.82 -1.7 1,126,515 - BWI76_RS05345 0.83 -3.2 1,126,553 - BWI76_RS05345 0.88 -2.4 1,126,555 + BWI76_RS05345 0.88 -1.2 1,126,659 + -1.4 1,126,677 + BWI76_RS05350 0.11 -3.7 1,126,695 - BWI76_RS05350 0.15 -4.0 1,126,751 + BWI76_RS05350 0.29 -3.4 1,126,759 - BWI76_RS05350 0.31 -6.1 1,126,954 + BWI76_RS05350 0.79 -1.9 1,126,962 - BWI76_RS05350 0.81 -3.1 1,126,979 + BWI76_RS05350 0.85 -2.6 1,126,986 + BWI76_RS05350 0.87 -1.7 1,126,986 + BWI76_RS05350 0.87 -2.0 1,126,986 + BWI76_RS05350 0.87 -2.5 1,126,989 - BWI76_RS05350 0.87 -5.0 1,127,008 - -3.3 1,127,138 - -2.4 1,127,155 + +0.4 1,127,163 - +0.5 1,127,214 + -0.3 1,127,221 - +1.1 1,127,301 - BWI76_RS05355 0.19 +1.3 1,127,301 - BWI76_RS05355 0.19 -2.4 1,127,302 - BWI76_RS05355 0.19 +0.9 1,127,542 + BWI76_RS05355 0.48 -1.6 1,127,690 + BWI76_RS05355 0.66 -1.1 1,127,693 - BWI76_RS05355 0.67 +0.5 1,127,698 - BWI76_RS05355 0.67 -1.0 1,127,698 - BWI76_RS05355 0.67 -0.4 1,127,892 + -0.5 1,127,945 - +0.2
Or see this region's nucleotide sequence