Experiment: KLEB14 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02305 and BWI76_RS02310 are separated by 67 nucleotides BWI76_RS02310 and BWI76_RS02315 are separated by 35 nucleotides BWI76_RS02315 and BWI76_RS02320 overlap by 25 nucleotides BWI76_RS02320 and BWI76_RS02325 are separated by 94 nucleotides
BWI76_RS02305: BWI76_RS02305 - hypothetical protein, at 473,077 to 475,164
_RS02305
BWI76_RS02310: BWI76_RS02310 - transcriptional regulator, at 475,232 to 475,807
_RS02310
BWI76_RS02315: BWI76_RS02315 - protein-disulfide reductase DsbD, at 475,843 to 477,600
_RS02315
BWI76_RS02320: BWI76_RS02320 - divalent ion tolerance protein CutA, at 477,576 to 477,899
_RS02320
BWI76_RS02325: BWI76_RS02325 - anaerobic C4-dicarboxylate transporter, at 477,994 to 479,295
_RS02325
Position (kb) 475
476
477
478 Strain fitness (log2 ratio) -2
-1
0
1
2 at 474.890 kb on + strand, within BWI76_RS02305 at 474.996 kb on - strand at 475.014 kb on + strand at 475.121 kb on + strand at 475.127 kb on - strand at 475.146 kb on + strand at 475.172 kb on - strand at 475.172 kb on - strand at 475.251 kb on + strand at 475.251 kb on + strand at 475.328 kb on - strand, within BWI76_RS02310 at 475.346 kb on + strand, within BWI76_RS02310 at 475.540 kb on + strand, within BWI76_RS02310 at 475.548 kb on - strand, within BWI76_RS02310 at 475.592 kb on - strand, within BWI76_RS02310 at 475.688 kb on - strand, within BWI76_RS02310 at 475.699 kb on + strand, within BWI76_RS02310 at 475.854 kb on - strand at 475.906 kb on - strand at 475.973 kb on - strand at 476.037 kb on + strand, within BWI76_RS02315 at 476.045 kb on - strand, within BWI76_RS02315 at 476.078 kb on - strand, within BWI76_RS02315 at 476.263 kb on - strand, within BWI76_RS02315 at 476.270 kb on + strand, within BWI76_RS02315 at 476.492 kb on + strand, within BWI76_RS02315 at 476.586 kb on + strand, within BWI76_RS02315 at 476.686 kb on + strand, within BWI76_RS02315 at 476.694 kb on - strand, within BWI76_RS02315 at 476.786 kb on - strand, within BWI76_RS02315 at 476.878 kb on + strand, within BWI76_RS02315 at 477.006 kb on - strand, within BWI76_RS02315 at 477.252 kb on + strand, within BWI76_RS02315 at 477.353 kb on - strand, within BWI76_RS02315 at 477.423 kb on + strand, within BWI76_RS02315 at 477.453 kb on + strand at 477.461 kb on - strand at 477.573 kb on - strand at 477.681 kb on + strand, within BWI76_RS02320 at 477.793 kb on - strand, within BWI76_RS02320 at 477.837 kb on + strand, within BWI76_RS02320 at 477.844 kb on - strand, within BWI76_RS02320 at 477.860 kb on + strand, within BWI76_RS02320 at 477.868 kb on - strand at 477.881 kb on - strand at 477.929 kb on + strand at 477.972 kb on - strand at 478.046 kb on - strand at 478.060 kb on + strand at 478.098 kb on + strand at 478.201 kb on - strand, within BWI76_RS02325 at 478.211 kb on - strand, within BWI76_RS02325 at 478.336 kb on + strand, within BWI76_RS02325 at 478.346 kb on + strand, within BWI76_RS02325 at 478.472 kb on + strand, within BWI76_RS02325 at 478.480 kb on - strand, within BWI76_RS02325 at 478.538 kb on - strand, within BWI76_RS02325 at 478.543 kb on + strand, within BWI76_RS02325 at 478.548 kb on + strand, within BWI76_RS02325 at 478.556 kb on - strand, within BWI76_RS02325
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB14 MOI 0.1 remove 474,890 + BWI76_RS02305 0.87 +0.0 474,996 - +0.3 475,014 + -0.5 475,121 + +1.5 475,127 - -0.4 475,146 + +1.8 475,172 - -0.8 475,172 - +0.4 475,251 + -0.1 475,251 + -2.2 475,328 - BWI76_RS02310 0.17 -0.4 475,346 + BWI76_RS02310 0.20 +1.8 475,540 + BWI76_RS02310 0.53 +0.6 475,548 - BWI76_RS02310 0.55 -0.1 475,592 - BWI76_RS02310 0.62 -0.3 475,688 - BWI76_RS02310 0.79 +0.7 475,699 + BWI76_RS02310 0.81 +0.5 475,854 - -1.1 475,906 - -0.5 475,973 - -0.6 476,037 + BWI76_RS02315 0.11 -0.5 476,045 - BWI76_RS02315 0.11 -1.0 476,078 - BWI76_RS02315 0.13 -0.3 476,263 - BWI76_RS02315 0.24 -1.7 476,270 + BWI76_RS02315 0.24 -1.2 476,492 + BWI76_RS02315 0.37 -1.5 476,586 + BWI76_RS02315 0.42 -0.4 476,686 + BWI76_RS02315 0.48 -0.8 476,694 - BWI76_RS02315 0.48 +2.5 476,786 - BWI76_RS02315 0.54 +0.3 476,878 + BWI76_RS02315 0.59 -1.5 477,006 - BWI76_RS02315 0.66 -2.6 477,252 + BWI76_RS02315 0.80 +0.6 477,353 - BWI76_RS02315 0.86 -0.7 477,423 + BWI76_RS02315 0.90 -1.2 477,453 + -2.3 477,461 - -0.8 477,573 - -0.6 477,681 + BWI76_RS02320 0.32 -0.4 477,793 - BWI76_RS02320 0.67 -0.2 477,837 + BWI76_RS02320 0.81 -1.9 477,844 - BWI76_RS02320 0.83 +0.3 477,860 + BWI76_RS02320 0.88 -0.4 477,868 - -0.5 477,881 - -0.5 477,929 + -0.4 477,972 - +0.1 478,046 - -0.1 478,060 + -0.6 478,098 + +0.2 478,201 - BWI76_RS02325 0.16 +0.6 478,211 - BWI76_RS02325 0.17 +0.3 478,336 + BWI76_RS02325 0.26 -0.8 478,346 + BWI76_RS02325 0.27 -0.8 478,472 + BWI76_RS02325 0.37 -0.0 478,480 - BWI76_RS02325 0.37 -0.9 478,538 - BWI76_RS02325 0.42 +0.4 478,543 + BWI76_RS02325 0.42 -0.9 478,548 + BWI76_RS02325 0.43 +1.0 478,556 - BWI76_RS02325 0.43 +2.3
Or see this region's nucleotide sequence