Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23960 and BWI76_RS23965 are separated by 90 nucleotides BWI76_RS23965 and BWI76_RS23970 are separated by 150 nucleotides BWI76_RS23970 and BWI76_RS23975 are separated by 188 nucleotides
BWI76_RS23960: BWI76_RS23960 - oxidative stress defense protein, at 5,001,381 to 5,002,148
_RS23960
BWI76_RS23965: BWI76_RS23965 - arginine exporter protein, at 5,002,239 to 5,002,874
_RS23965
BWI76_RS23970: BWI76_RS23970 - mechanosensitive ion channel protein MscS, at 5,003,025 to 5,003,882
_RS23970
BWI76_RS23975: BWI76_RS23975 - class II fructose-bisphosphate aldolase, at 5,004,071 to 5,005,150
_RS23975
Position (kb)
5003
5004 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5002.184 kb on - strand at 5002.252 kb on + strand at 5002.355 kb on + strand, within BWI76_RS23965 at 5002.435 kb on - strand, within BWI76_RS23965 at 5003.009 kb on + strand at 5003.220 kb on - strand, within BWI76_RS23970 at 5003.415 kb on + strand, within BWI76_RS23970 at 5003.415 kb on + strand, within BWI76_RS23970 at 5003.538 kb on - strand, within BWI76_RS23970 at 5003.800 kb on + strand at 5003.813 kb on - strand at 5003.834 kb on + strand at 5003.936 kb on - strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.952 kb on - strand at 5003.952 kb on - strand at 5003.952 kb on - strand at 5003.955 kb on + strand at 5003.957 kb on + strand at 5003.963 kb on - strand at 5003.963 kb on - strand at 5003.987 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 5,002,184 - +0.7 5,002,252 + -0.9 5,002,355 + BWI76_RS23965 0.18 -0.2 5,002,435 - BWI76_RS23965 0.31 +0.2 5,003,009 + -0.7 5,003,220 - BWI76_RS23970 0.23 +0.7 5,003,415 + BWI76_RS23970 0.45 -1.3 5,003,415 + BWI76_RS23970 0.45 -2.0 5,003,538 - BWI76_RS23970 0.60 +0.0 5,003,800 + -1.1 5,003,813 - -2.9 5,003,834 + +0.5 5,003,936 - -0.3 5,003,944 + -1.1 5,003,944 + -1.8 5,003,944 + -0.0 5,003,944 + +0.7 5,003,944 + -1.9 5,003,944 + +3.4 5,003,952 - +0.4 5,003,952 - -0.0 5,003,952 - -0.6 5,003,955 + +0.0 5,003,957 + +0.1 5,003,963 - -1.9 5,003,963 - -0.2 5,003,987 + -0.2
Or see this region's nucleotide sequence