Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20045

Experiment: KLEB13 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20035 and BWI76_RS20040 are separated by 15 nucleotidesBWI76_RS20040 and BWI76_RS20045 are separated by 11 nucleotidesBWI76_RS20045 and BWI76_RS20050 are separated by 105 nucleotidesBWI76_RS20050 and BWI76_RS20055 overlap by 24 nucleotides BWI76_RS20035: BWI76_RS20035 - hypothetical protein, at 4,197,551 to 4,198,531 _RS20035 BWI76_RS20040: BWI76_RS20040 - tricarboxylic transport membrane protein, at 4,198,547 to 4,198,978 _RS20040 BWI76_RS20045: BWI76_RS20045 - hypothetical protein, at 4,198,990 to 4,200,510 _RS20045 BWI76_RS20050: BWI76_RS20050 - hypothetical protein, at 4,200,616 to 4,201,239 _RS20050 BWI76_RS20055: BWI76_RS20055 - 2-succinylbenzoate-CoA ligase, at 4,201,216 to 4,202,607 _RS20055 Position (kb) 4198 4199 4200 4201Strain fitness (log2 ratio) -2 -1 0 1at 4198.263 kb on + strand, within BWI76_RS20035at 4198.393 kb on + strand, within BWI76_RS20035at 4198.677 kb on + strand, within BWI76_RS20040at 4198.697 kb on + strand, within BWI76_RS20040at 4198.737 kb on - strand, within BWI76_RS20040at 4198.909 kb on - strand, within BWI76_RS20040at 4199.204 kb on - strand, within BWI76_RS20045at 4199.237 kb on + strand, within BWI76_RS20045at 4199.343 kb on - strand, within BWI76_RS20045at 4199.825 kb on + strand, within BWI76_RS20045at 4200.145 kb on - strand, within BWI76_RS20045at 4200.169 kb on - strand, within BWI76_RS20045at 4200.844 kb on + strand, within BWI76_RS20050

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1
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4,198,263 + BWI76_RS20035 0.73 +1.1
4,198,393 + BWI76_RS20035 0.86 +1.4
4,198,677 + BWI76_RS20040 0.30 -0.5
4,198,697 + BWI76_RS20040 0.35 -1.3
4,198,737 - BWI76_RS20040 0.44 +1.5
4,198,909 - BWI76_RS20040 0.84 +0.3
4,199,204 - BWI76_RS20045 0.14 +0.2
4,199,237 + BWI76_RS20045 0.16 -0.6
4,199,343 - BWI76_RS20045 0.23 -0.5
4,199,825 + BWI76_RS20045 0.55 -0.7
4,200,145 - BWI76_RS20045 0.76 -0.5
4,200,169 - BWI76_RS20045 0.78 -2.1
4,200,844 + BWI76_RS20050 0.37 -0.3

Or see this region's nucleotide sequence