Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18220 and BWI76_RS18225 are separated by 362 nucleotides BWI76_RS18225 and BWI76_RS18230 are separated by 28 nucleotides BWI76_RS18230 and BWI76_RS18235 overlap by 26 nucleotides BWI76_RS18235 and BWI76_RS18240 are separated by 159 nucleotides
BWI76_RS18220: BWI76_RS18220 - MFS transporter, at 3,785,287 to 3,786,870
_RS18220
BWI76_RS18225: BWI76_RS18225 - universal stress protein C, at 3,787,233 to 3,787,661
_RS18225
BWI76_RS18230: BWI76_RS18230 - trehalose-6-phosphate synthase, at 3,787,690 to 3,789,114
_RS18230
BWI76_RS18235: BWI76_RS18235 - trehalose-phosphatase, at 3,789,089 to 3,789,892
_RS18235
BWI76_RS18240: BWI76_RS18240 - L-arabinose ABC transporter permease AraH, at 3,790,052 to 3,791,032
_RS18240
Position (kb)
3787
3788
3789
3790 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3787.542 kb on + strand, within BWI76_RS18225 at 3787.542 kb on + strand, within BWI76_RS18225 at 3787.567 kb on + strand, within BWI76_RS18225 at 3787.672 kb on + strand at 3788.197 kb on + strand, within BWI76_RS18230 at 3788.323 kb on - strand, within BWI76_RS18230 at 3788.400 kb on + strand, within BWI76_RS18230 at 3788.412 kb on + strand, within BWI76_RS18230 at 3789.109 kb on + strand at 3789.429 kb on + strand, within BWI76_RS18235 at 3789.542 kb on - strand, within BWI76_RS18235 at 3789.645 kb on - strand, within BWI76_RS18235 at 3789.754 kb on + strand, within BWI76_RS18235 at 3789.811 kb on - strand, within BWI76_RS18235 at 3789.961 kb on - strand at 3790.092 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 3,787,542 + BWI76_RS18225 0.72 -0.9 3,787,542 + BWI76_RS18225 0.72 +1.2 3,787,567 + BWI76_RS18225 0.78 +0.8 3,787,672 + +0.5 3,788,197 + BWI76_RS18230 0.36 -1.2 3,788,323 - BWI76_RS18230 0.44 -0.0 3,788,400 + BWI76_RS18230 0.50 -1.6 3,788,412 + BWI76_RS18230 0.51 -0.6 3,789,109 + +2.3 3,789,429 + BWI76_RS18235 0.42 -0.7 3,789,542 - BWI76_RS18235 0.56 +0.9 3,789,645 - BWI76_RS18235 0.69 -1.9 3,789,754 + BWI76_RS18235 0.83 -3.0 3,789,811 - BWI76_RS18235 0.90 -0.6 3,789,961 - +4.7 3,790,092 - -0.7
Or see this region's nucleotide sequence