Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18230

Experiment: KLEB13 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18220 and BWI76_RS18225 are separated by 362 nucleotidesBWI76_RS18225 and BWI76_RS18230 are separated by 28 nucleotidesBWI76_RS18230 and BWI76_RS18235 overlap by 26 nucleotidesBWI76_RS18235 and BWI76_RS18240 are separated by 159 nucleotides BWI76_RS18220: BWI76_RS18220 - MFS transporter, at 3,785,287 to 3,786,870 _RS18220 BWI76_RS18225: BWI76_RS18225 - universal stress protein C, at 3,787,233 to 3,787,661 _RS18225 BWI76_RS18230: BWI76_RS18230 - trehalose-6-phosphate synthase, at 3,787,690 to 3,789,114 _RS18230 BWI76_RS18235: BWI76_RS18235 - trehalose-phosphatase, at 3,789,089 to 3,789,892 _RS18235 BWI76_RS18240: BWI76_RS18240 - L-arabinose ABC transporter permease AraH, at 3,790,052 to 3,791,032 _RS18240 Position (kb) 3787 3788 3789 3790Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3787.542 kb on + strand, within BWI76_RS18225at 3787.542 kb on + strand, within BWI76_RS18225at 3787.567 kb on + strand, within BWI76_RS18225at 3787.672 kb on + strandat 3788.197 kb on + strand, within BWI76_RS18230at 3788.323 kb on - strand, within BWI76_RS18230at 3788.400 kb on + strand, within BWI76_RS18230at 3788.412 kb on + strand, within BWI76_RS18230at 3789.109 kb on + strandat 3789.429 kb on + strand, within BWI76_RS18235at 3789.542 kb on - strand, within BWI76_RS18235at 3789.645 kb on - strand, within BWI76_RS18235at 3789.754 kb on + strand, within BWI76_RS18235at 3789.811 kb on - strand, within BWI76_RS18235at 3789.961 kb on - strandat 3790.092 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1
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3,787,542 + BWI76_RS18225 0.72 -0.9
3,787,542 + BWI76_RS18225 0.72 +1.2
3,787,567 + BWI76_RS18225 0.78 +0.8
3,787,672 + +0.5
3,788,197 + BWI76_RS18230 0.36 -1.2
3,788,323 - BWI76_RS18230 0.44 -0.0
3,788,400 + BWI76_RS18230 0.50 -1.6
3,788,412 + BWI76_RS18230 0.51 -0.6
3,789,109 + +2.3
3,789,429 + BWI76_RS18235 0.42 -0.7
3,789,542 - BWI76_RS18235 0.56 +0.9
3,789,645 - BWI76_RS18235 0.69 -1.9
3,789,754 + BWI76_RS18235 0.83 -3.0
3,789,811 - BWI76_RS18235 0.90 -0.6
3,789,961 - +4.7
3,790,092 - -0.7

Or see this region's nucleotide sequence