Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17805 and BWI76_RS17810 are separated by 43 nucleotides BWI76_RS17810 and BWI76_RS17815 are separated by 67 nucleotides BWI76_RS17815 and BWI76_RS17820 are separated by 132 nucleotides BWI76_RS17820 and BWI76_RS17825 are separated by 164 nucleotides
BWI76_RS17805: BWI76_RS17805 - membrane protein, at 3,701,023 to 3,701,607
_RS17805
BWI76_RS17810: BWI76_RS17810 - tRNA N6-adenosine(37)-N6-threonylcarbamoyltransferase complex dimerization subunit TsaB, at 3,701,651 to 3,702,346
_RS17810
BWI76_RS17815: BWI76_RS17815 - ATP-dependent helicase, at 3,702,414 to 3,704,324
_RS17815
BWI76_RS17820: BWI76_RS17820 - hypothetical protein, at 3,704,457 to 3,704,801
_RS17820
BWI76_RS17825: BWI76_RS17825 - alginate lyase, at 3,704,966 to 3,706,105
_RS17825
Position (kb) 3702
3703
3704
3705 Strain fitness (log2 ratio) -1
0
1
2
3 at 3701.461 kb on - strand, within BWI76_RS17805 at 3702.456 kb on + strand at 3702.587 kb on - strand at 3702.590 kb on + strand at 3702.598 kb on - strand at 3702.866 kb on - strand, within BWI76_RS17815 at 3702.902 kb on - strand, within BWI76_RS17815 at 3702.917 kb on + strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.948 kb on - strand, within BWI76_RS17815 at 3702.990 kb on - strand, within BWI76_RS17815 at 3703.026 kb on - strand, within BWI76_RS17815 at 3703.037 kb on - strand, within BWI76_RS17815 at 3703.093 kb on - strand, within BWI76_RS17815 at 3703.847 kb on - strand, within BWI76_RS17815 at 3703.980 kb on - strand, within BWI76_RS17815 at 3703.993 kb on - strand, within BWI76_RS17815 at 3703.993 kb on - strand, within BWI76_RS17815 at 3704.266 kb on + strand at 3704.530 kb on - strand, within BWI76_RS17820 at 3704.724 kb on + strand, within BWI76_RS17820 at 3704.796 kb on + strand at 3704.796 kb on + strand at 3704.804 kb on - strand at 3704.804 kb on - strand at 3704.828 kb on - strand at 3705.108 kb on + strand, within BWI76_RS17825 at 3705.136 kb on - strand, within BWI76_RS17825
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 3,701,461 - BWI76_RS17805 0.75 -1.7 3,702,456 + -0.2 3,702,587 - -0.0 3,702,590 + -1.1 3,702,598 - +0.3 3,702,866 - BWI76_RS17815 0.24 -0.1 3,702,902 - BWI76_RS17815 0.26 -1.0 3,702,917 + BWI76_RS17815 0.26 -0.1 3,702,925 - BWI76_RS17815 0.27 -1.1 3,702,925 - BWI76_RS17815 0.27 +0.5 3,702,925 - BWI76_RS17815 0.27 +1.0 3,702,925 - BWI76_RS17815 0.27 -0.9 3,702,948 - BWI76_RS17815 0.28 -0.4 3,702,990 - BWI76_RS17815 0.30 +0.7 3,703,026 - BWI76_RS17815 0.32 -0.0 3,703,037 - BWI76_RS17815 0.33 +0.2 3,703,093 - BWI76_RS17815 0.36 +3.5 3,703,847 - BWI76_RS17815 0.75 +0.5 3,703,980 - BWI76_RS17815 0.82 -1.2 3,703,993 - BWI76_RS17815 0.83 +0.6 3,703,993 - BWI76_RS17815 0.83 -0.2 3,704,266 + -0.1 3,704,530 - BWI76_RS17820 0.21 +0.1 3,704,724 + BWI76_RS17820 0.77 -0.4 3,704,796 + +0.1 3,704,796 + +1.2 3,704,804 - +0.1 3,704,804 - +0.3 3,704,828 - +0.4 3,705,108 + BWI76_RS17825 0.12 +0.2 3,705,136 - BWI76_RS17825 0.15 -1.2
Or see this region's nucleotide sequence