Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16905 and BWI76_RS16910 overlap by 8 nucleotides BWI76_RS16910 and BWI76_RS16915 are separated by 9 nucleotides BWI76_RS16915 and BWI76_RS16920 are separated by 17 nucleotides BWI76_RS16920 and BWI76_RS16925 are separated by 1 nucleotides
BWI76_RS16905: BWI76_RS16905 - acetaldehyde dehydrogenase (acetylating), at 3,505,611 to 3,506,561
_RS16905
BWI76_RS16910: BWI76_RS16910 - 2-keto-4-pentenoate hydratase, at 3,506,554 to 3,507,360
_RS16910
BWI76_RS16915: BWI76_RS16915 - alpha/beta hydrolase fold protein, at 3,507,370 to 3,508,236
_RS16915
BWI76_RS16920: BWI76_RS16920 - 3-(2,3-dihydroxyphenyl)propionate dioxygenase, at 3,508,254 to 3,509,198
_RS16920
BWI76_RS16925: BWI76_RS16925 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 3,509,200 to 3,510,867
_RS16925
Position (kb)
3507
3508
3509 Strain fitness (log2 ratio)
-1
0
1
2 at 3506.575 kb on - strand at 3506.594 kb on - strand at 3506.986 kb on - strand, within BWI76_RS16910 at 3507.250 kb on - strand, within BWI76_RS16910 at 3507.409 kb on + strand at 3507.410 kb on - strand at 3507.823 kb on - strand, within BWI76_RS16915 at 3507.854 kb on + strand, within BWI76_RS16915 at 3508.140 kb on + strand, within BWI76_RS16915 at 3508.418 kb on + strand, within BWI76_RS16920 at 3508.568 kb on + strand, within BWI76_RS16920 at 3509.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 3,506,575 - +1.2 3,506,594 - -0.8 3,506,986 - BWI76_RS16910 0.54 +1.9 3,507,250 - BWI76_RS16910 0.86 -0.5 3,507,409 + +2.5 3,507,410 - +1.3 3,507,823 - BWI76_RS16915 0.52 -0.1 3,507,854 + BWI76_RS16915 0.56 +0.6 3,508,140 + BWI76_RS16915 0.89 +1.1 3,508,418 + BWI76_RS16920 0.17 +1.1 3,508,568 + BWI76_RS16920 0.33 +1.1 3,509,124 + +1.7
Or see this region's nucleotide sequence