Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16860 and BWI76_RS16865 are separated by 53 nucleotides BWI76_RS16865 and BWI76_RS16870 are separated by 145 nucleotides
BWI76_RS16860: BWI76_RS16860 - acyl-CoA dehydrogenase, at 3,496,352 to 3,497,440
_RS16860
BWI76_RS16865: BWI76_RS16865 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase-related flavin-dependent oxidoreductase, at 3,497,494 to 3,498,579
_RS16865
BWI76_RS16870: BWI76_RS16870 - LysR family transcriptional regulator, at 3,498,725 to 3,499,618
_RS16870
Position (kb)
3497
3498
3499 Strain fitness (log2 ratio)
-1
0
1
2 at 3496.666 kb on + strand, within BWI76_RS16860 at 3496.720 kb on - strand, within BWI76_RS16860 at 3497.655 kb on + strand, within BWI76_RS16865 at 3497.663 kb on + strand, within BWI76_RS16865 at 3497.663 kb on - strand, within BWI76_RS16865 at 3497.738 kb on + strand, within BWI76_RS16865 at 3498.029 kb on + strand, within BWI76_RS16865 at 3498.154 kb on + strand, within BWI76_RS16865 at 3498.173 kb on + strand at 3498.181 kb on - strand, within BWI76_RS16865 at 3498.647 kb on - strand at 3498.975 kb on + strand, within BWI76_RS16870 at 3498.976 kb on - strand, within BWI76_RS16870 at 3499.376 kb on - strand, within BWI76_RS16870
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 3,496,666 + BWI76_RS16860 0.29 +1.6 3,496,720 - BWI76_RS16860 0.34 -1.1 3,497,655 + BWI76_RS16865 0.15 -1.0 3,497,663 + BWI76_RS16865 0.16 +0.1 3,497,663 - BWI76_RS16865 0.16 +0.3 3,497,738 + BWI76_RS16865 0.22 +0.7 3,498,029 + BWI76_RS16865 0.49 +1.2 3,498,154 + BWI76_RS16865 0.61 +0.8 3,498,173 + +1.7 3,498,181 - BWI76_RS16865 0.63 +1.9 3,498,647 - -0.7 3,498,975 + BWI76_RS16870 0.28 -0.7 3,498,976 - BWI76_RS16870 0.28 +0.2 3,499,376 - BWI76_RS16870 0.73 +0.5
Or see this region's nucleotide sequence