Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14710 and BWI76_RS14715 are separated by 49 nucleotides BWI76_RS14715 and BWI76_RS14720 overlap by 20 nucleotides BWI76_RS14720 and BWI76_RS14725 are separated by 87 nucleotides BWI76_RS14725 and BWI76_RS14730 are separated by 61 nucleotides
BWI76_RS14710: BWI76_RS14710 - FAD-dependent oxidoreductase, at 3,088,960 to 3,090,282
_RS14710
BWI76_RS14715: BWI76_RS14715 - type I methionyl aminopeptidase, at 3,090,332 to 3,091,111
_RS14715
BWI76_RS14720: BWI76_RS14720 - hypothetical protein, at 3,091,092 to 3,091,310
_RS14720
BWI76_RS14725: BWI76_RS14725 - HxlR family transcriptional regulator, at 3,091,398 to 3,091,865
_RS14725
BWI76_RS14730: BWI76_RS14730 - 4-oxalocrotonate tautomerase, at 3,091,927 to 3,092,163
_RS14730
Position (kb)
3090
3091
3092 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3089.405 kb on + strand, within BWI76_RS14710 at 3089.424 kb on + strand, within BWI76_RS14710 at 3089.836 kb on - strand, within BWI76_RS14710 at 3090.044 kb on - strand, within BWI76_RS14710 at 3090.516 kb on - strand, within BWI76_RS14715 at 3090.652 kb on - strand, within BWI76_RS14715 at 3090.693 kb on + strand, within BWI76_RS14715 at 3091.003 kb on - strand, within BWI76_RS14715 at 3091.020 kb on - strand, within BWI76_RS14715 at 3091.412 kb on + strand at 3091.412 kb on + strand at 3091.420 kb on - strand at 3091.420 kb on - strand at 3091.420 kb on - strand at 3091.420 kb on - strand at 3091.496 kb on - strand, within BWI76_RS14725 at 3091.642 kb on - strand, within BWI76_RS14725 at 3091.752 kb on + strand, within BWI76_RS14725 at 3091.752 kb on + strand, within BWI76_RS14725 at 3091.760 kb on - strand, within BWI76_RS14725 at 3091.942 kb on + strand at 3092.064 kb on - strand, within BWI76_RS14730 at 3092.066 kb on + strand, within BWI76_RS14730 at 3092.068 kb on - strand, within BWI76_RS14730 at 3092.081 kb on + strand, within BWI76_RS14730
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 3,089,405 + BWI76_RS14710 0.34 +0.4 3,089,424 + BWI76_RS14710 0.35 -0.1 3,089,836 - BWI76_RS14710 0.66 +0.2 3,090,044 - BWI76_RS14710 0.82 -0.9 3,090,516 - BWI76_RS14715 0.24 -1.7 3,090,652 - BWI76_RS14715 0.41 +0.8 3,090,693 + BWI76_RS14715 0.46 -0.9 3,091,003 - BWI76_RS14715 0.86 -2.0 3,091,020 - BWI76_RS14715 0.88 -0.7 3,091,412 + -0.1 3,091,412 + +0.9 3,091,420 - -0.1 3,091,420 - +0.7 3,091,420 - -0.8 3,091,420 - +3.0 3,091,496 - BWI76_RS14725 0.21 +0.5 3,091,642 - BWI76_RS14725 0.52 +0.3 3,091,752 + BWI76_RS14725 0.76 -1.5 3,091,752 + BWI76_RS14725 0.76 -2.1 3,091,760 - BWI76_RS14725 0.77 +0.6 3,091,942 + -1.5 3,092,064 - BWI76_RS14730 0.58 -2.3 3,092,066 + BWI76_RS14730 0.59 -0.2 3,092,068 - BWI76_RS14730 0.59 +0.0 3,092,081 + BWI76_RS14730 0.65 -0.0
Or see this region's nucleotide sequence