Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14150

Experiment: KLEB13 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14145 and BWI76_RS14150 overlap by 17 nucleotidesBWI76_RS14150 and BWI76_RS14155 are separated by 3 nucleotides BWI76_RS14145: BWI76_RS14145 - dihydrolipoyl dehydrogenase, at 2,958,605 to 2,960,002 _RS14145 BWI76_RS14150: BWI76_RS14150 - dihydrolipoyllysine-residue acetyltransferase, at 2,959,986 to 2,961,515 _RS14150 BWI76_RS14155: BWI76_RS14155 - transketolase, at 2,961,519 to 2,962,538 _RS14155 Position (kb) 2959 2960 2961 2962Strain fitness (log2 ratio) -1 0 1 2at 2959.053 kb on + strand, within BWI76_RS14145at 2959.278 kb on + strand, within BWI76_RS14145at 2959.758 kb on - strand, within BWI76_RS14145at 2960.174 kb on - strand, within BWI76_RS14150at 2960.241 kb on - strand, within BWI76_RS14150at 2961.052 kb on - strand, within BWI76_RS14150at 2961.084 kb on + strand, within BWI76_RS14150at 2961.119 kb on + strand, within BWI76_RS14150at 2961.200 kb on + strand, within BWI76_RS14150at 2961.600 kb on - strandat 2961.600 kb on - strandat 2961.602 kb on + strandat 2962.064 kb on + strand, within BWI76_RS14155at 2962.351 kb on - strand, within BWI76_RS14155at 2962.356 kb on - strand, within BWI76_RS14155at 2962.457 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1
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2,959,053 + BWI76_RS14145 0.32 -1.7
2,959,278 + BWI76_RS14145 0.48 -1.1
2,959,758 - BWI76_RS14145 0.82 +2.0
2,960,174 - BWI76_RS14150 0.12 -0.3
2,960,241 - BWI76_RS14150 0.17 -1.3
2,961,052 - BWI76_RS14150 0.70 -0.8
2,961,084 + BWI76_RS14150 0.72 -1.4
2,961,119 + BWI76_RS14150 0.74 -0.6
2,961,200 + BWI76_RS14150 0.79 -0.8
2,961,600 - -0.1
2,961,600 - -0.1
2,961,602 + +0.1
2,962,064 + BWI76_RS14155 0.53 +0.2
2,962,351 - BWI76_RS14155 0.82 -0.2
2,962,356 - BWI76_RS14155 0.82 -0.7
2,962,457 - +0.1

Or see this region's nucleotide sequence