Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13430 and BWI76_RS13435 are separated by 52 nucleotides BWI76_RS13435 and BWI76_RS13440 are separated by 196 nucleotides BWI76_RS13440 and BWI76_RS13445 are separated by 72 nucleotides BWI76_RS13445 and BWI76_RS13450 are separated by 80 nucleotides
BWI76_RS13430: BWI76_RS13430 - hypothetical protein, at 2,802,498 to 2,802,782
_RS13430
BWI76_RS13435: BWI76_RS13435 - hypothetical protein, at 2,802,835 to 2,802,927
_RS13435
BWI76_RS13440: BWI76_RS13440 - DUF2566 domain-containing protein, at 2,803,124 to 2,803,297
_RS13440
BWI76_RS13445: BWI76_RS13445 - alpha/beta hydrolase, at 2,803,370 to 2,804,140
_RS13445
BWI76_RS13450: BWI76_RS13450 - acyl-CoA thioesterase, at 2,804,221 to 2,805,477
_RS13450
Position (kb)
2803
2804
2805 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2802.405 kb on + strand at 2802.406 kb on - strand at 2802.406 kb on - strand at 2802.455 kb on - strand at 2802.470 kb on - strand at 2802.482 kb on - strand at 2802.658 kb on + strand, within BWI76_RS13430 at 2802.898 kb on - strand, within BWI76_RS13435 at 2802.957 kb on + strand at 2802.968 kb on + strand at 2803.061 kb on + strand at 2803.388 kb on + strand at 2803.593 kb on - strand, within BWI76_RS13445 at 2803.782 kb on - strand, within BWI76_RS13445 at 2804.244 kb on - strand at 2804.264 kb on - strand at 2804.306 kb on + strand at 2804.578 kb on + strand, within BWI76_RS13450 at 2804.645 kb on + strand, within BWI76_RS13450 at 2804.797 kb on + strand, within BWI76_RS13450 at 2804.846 kb on - strand, within BWI76_RS13450 at 2804.846 kb on - strand, within BWI76_RS13450
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 2,802,405 + -1.2 2,802,406 - -3.9 2,802,406 - +0.4 2,802,455 - +1.8 2,802,470 - -0.6 2,802,482 - -1.0 2,802,658 + BWI76_RS13430 0.56 +1.8 2,802,898 - BWI76_RS13435 0.68 +1.3 2,802,957 + -0.5 2,802,968 + -0.5 2,803,061 + -2.3 2,803,388 + +0.7 2,803,593 - BWI76_RS13445 0.29 -2.0 2,803,782 - BWI76_RS13445 0.53 -2.4 2,804,244 - +0.1 2,804,264 - +1.1 2,804,306 + +1.1 2,804,578 + BWI76_RS13450 0.28 +1.8 2,804,645 + BWI76_RS13450 0.34 +0.9 2,804,797 + BWI76_RS13450 0.46 -2.7 2,804,846 - BWI76_RS13450 0.50 -0.8 2,804,846 - BWI76_RS13450 0.50 -0.8
Or see this region's nucleotide sequence