Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12735

Experiment: KLEB13 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12730 and BWI76_RS12735 are separated by 623 nucleotidesBWI76_RS12735 and BWI76_RS12740 are separated by 427 nucleotidesBWI76_RS12740 and BWI76_RS12745 are separated by 76 nucleotides BWI76_RS12730: BWI76_RS12730 - pyruvate:ferredoxin (flavodoxin) oxidoreductase, at 2,653,158 to 2,656,685 _RS12730 BWI76_RS12735: BWI76_RS12735 - phage repressor protein, at 2,657,309 to 2,657,845 _RS12735 BWI76_RS12740: BWI76_RS12740 - hypothetical protein, at 2,658,273 to 2,658,635 _RS12740 BWI76_RS12745: BWI76_RS12745 - putative prophage membrane protein, at 2,658,712 to 2,659,104 _RS12745 Position (kb) 2657 2658Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2656.757 kb on + strandat 2656.761 kb on - strandat 2656.952 kb on + strandat 2657.221 kb on + strandat 2657.268 kb on + strandat 2657.276 kb on - strandat 2657.441 kb on + strand, within BWI76_RS12735at 2657.759 kb on + strand, within BWI76_RS12735at 2657.826 kb on + strandat 2658.223 kb on - strandat 2658.237 kb on + strandat 2658.264 kb on - strandat 2658.264 kb on - strandat 2658.358 kb on + strand, within BWI76_RS12740at 2658.361 kb on - strand, within BWI76_RS12740at 2658.366 kb on - strand, within BWI76_RS12740at 2658.366 kb on - strand, within BWI76_RS12740at 2658.377 kb on + strand, within BWI76_RS12740at 2658.385 kb on - strand, within BWI76_RS12740at 2658.398 kb on + strand, within BWI76_RS12740at 2658.398 kb on + strand, within BWI76_RS12740at 2658.404 kb on + strand, within BWI76_RS12740at 2658.406 kb on - strand, within BWI76_RS12740at 2658.406 kb on - strand, within BWI76_RS12740at 2658.406 kb on - strandat 2658.412 kb on - strand, within BWI76_RS12740at 2658.412 kb on - strand, within BWI76_RS12740at 2658.417 kb on + strand, within BWI76_RS12740at 2658.419 kb on + strand, within BWI76_RS12740at 2658.419 kb on + strand, within BWI76_RS12740at 2658.422 kb on - strand, within BWI76_RS12740at 2658.425 kb on - strand, within BWI76_RS12740at 2658.427 kb on - strand, within BWI76_RS12740at 2658.427 kb on - strand, within BWI76_RS12740at 2658.430 kb on + strand, within BWI76_RS12740at 2658.430 kb on + strand, within BWI76_RS12740at 2658.430 kb on + strand, within BWI76_RS12740at 2658.430 kb on + strand, within BWI76_RS12740at 2658.436 kb on - strand, within BWI76_RS12740at 2658.438 kb on - strand, within BWI76_RS12740at 2658.438 kb on - strand, within BWI76_RS12740at 2658.438 kb on - strand, within BWI76_RS12740at 2658.438 kb on - strand, within BWI76_RS12740at 2658.438 kb on - strand, within BWI76_RS12740at 2658.478 kb on - strand, within BWI76_RS12740at 2658.548 kb on + strand, within BWI76_RS12740at 2658.556 kb on - strand, within BWI76_RS12740at 2658.619 kb on + strandat 2658.631 kb on + strandat 2658.638 kb on + strandat 2658.646 kb on - strandat 2658.689 kb on - strandat 2658.696 kb on + strandat 2658.704 kb on - strandat 2658.708 kb on + strandat 2658.714 kb on - strandat 2658.714 kb on - strandat 2658.716 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1
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2,656,757 + -0.2
2,656,761 - +0.9
2,656,952 + +0.2
2,657,221 + +0.3
2,657,268 + +1.3
2,657,276 - +0.1
2,657,441 + BWI76_RS12735 0.25 -1.3
2,657,759 + BWI76_RS12735 0.84 -1.5
2,657,826 + +1.8
2,658,223 - +0.8
2,658,237 + -2.2
2,658,264 - -0.0
2,658,264 - +1.1
2,658,358 + BWI76_RS12740 0.23 -0.5
2,658,361 - BWI76_RS12740 0.24 -0.1
2,658,366 - BWI76_RS12740 0.26 +0.3
2,658,366 - BWI76_RS12740 0.26 +0.4
2,658,377 + BWI76_RS12740 0.29 -2.7
2,658,385 - BWI76_RS12740 0.31 +0.1
2,658,398 + BWI76_RS12740 0.34 +1.1
2,658,398 + BWI76_RS12740 0.34 -1.4
2,658,404 + BWI76_RS12740 0.36 -0.6
2,658,406 - BWI76_RS12740 0.37 +1.8
2,658,406 - BWI76_RS12740 0.37 +3.1
2,658,406 - -0.5
2,658,412 - BWI76_RS12740 0.38 +0.1
2,658,412 - BWI76_RS12740 0.38 -0.7
2,658,417 + BWI76_RS12740 0.40 +2.1
2,658,419 + BWI76_RS12740 0.40 -1.5
2,658,419 + BWI76_RS12740 0.40 -1.3
2,658,422 - BWI76_RS12740 0.41 -1.1
2,658,425 - BWI76_RS12740 0.42 -0.2
2,658,427 - BWI76_RS12740 0.42 +0.1
2,658,427 - BWI76_RS12740 0.42 +0.3
2,658,430 + BWI76_RS12740 0.43 -2.9
2,658,430 + BWI76_RS12740 0.43 +0.8
2,658,430 + BWI76_RS12740 0.43 -1.7
2,658,430 + BWI76_RS12740 0.43 -0.2
2,658,436 - BWI76_RS12740 0.45 -1.7
2,658,438 - BWI76_RS12740 0.45 -1.6
2,658,438 - BWI76_RS12740 0.45 +0.7
2,658,438 - BWI76_RS12740 0.45 +1.0
2,658,438 - BWI76_RS12740 0.45 +0.7
2,658,438 - BWI76_RS12740 0.45 +0.3
2,658,478 - BWI76_RS12740 0.56 -0.6
2,658,548 + BWI76_RS12740 0.76 -0.8
2,658,556 - BWI76_RS12740 0.78 +2.3
2,658,619 + -1.7
2,658,631 + +1.8
2,658,638 + +0.8
2,658,646 - +0.6
2,658,689 - +0.3
2,658,696 + +3.4
2,658,704 - -1.5
2,658,708 + -0.1
2,658,714 - +1.8
2,658,714 - +0.3
2,658,716 - -0.5

Or see this region's nucleotide sequence