Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11230 and BWI76_RS11235 are separated by 28 nucleotides BWI76_RS11235 and BWI76_RS11240 are separated by 346 nucleotides BWI76_RS11240 and BWI76_RS11245 overlap by 4 nucleotides BWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotides
BWI76_RS11230: BWI76_RS11230 - NAD-dependent deacylase, at 2,369,681 to 2,370,526
_RS11230
BWI76_RS11235: BWI76_RS11235 - hypothetical protein, at 2,370,555 to 2,370,860
_RS11235
BWI76_RS11240: BWI76_RS11240 - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 2,371,207 to 2,372,253
_RS11240
BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035
_RS11245
BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889
_RS11250
Position (kb)
2371
2372
2373 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2370.211 kb on - strand, within BWI76_RS11230 at 2370.335 kb on - strand, within BWI76_RS11230 at 2370.442 kb on + strand at 2370.552 kb on + strand at 2370.560 kb on - strand at 2370.623 kb on + strand, within BWI76_RS11235 at 2370.904 kb on + strand at 2371.030 kb on + strand at 2371.166 kb on - strand at 2371.571 kb on + strand, within BWI76_RS11240 at 2371.715 kb on + strand, within BWI76_RS11240 at 2371.725 kb on + strand, within BWI76_RS11240 at 2371.762 kb on - strand, within BWI76_RS11240 at 2371.867 kb on - strand, within BWI76_RS11240 at 2372.461 kb on - strand, within BWI76_RS11245 at 2372.599 kb on - strand, within BWI76_RS11245 at 2372.922 kb on + strand, within BWI76_RS11245
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 2,370,211 - BWI76_RS11230 0.63 -0.3 2,370,335 - BWI76_RS11230 0.77 +1.5 2,370,442 + -0.4 2,370,552 + +0.6 2,370,560 - -0.1 2,370,623 + BWI76_RS11235 0.22 -1.1 2,370,904 + -2.5 2,371,030 + -0.1 2,371,166 - -0.7 2,371,571 + BWI76_RS11240 0.35 -2.6 2,371,715 + BWI76_RS11240 0.49 -2.8 2,371,725 + BWI76_RS11240 0.49 -3.8 2,371,762 - BWI76_RS11240 0.53 -0.6 2,371,867 - BWI76_RS11240 0.63 -0.4 2,372,461 - BWI76_RS11245 0.27 -0.4 2,372,599 - BWI76_RS11245 0.44 -0.3 2,372,922 + BWI76_RS11245 0.85 -0.1
Or see this region's nucleotide sequence