Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08580 and BWI76_RS08585 are separated by 35 nucleotides BWI76_RS08585 and BWI76_RS08590 overlap by 4 nucleotides BWI76_RS08590 and BWI76_RS08595 are separated by 118 nucleotides BWI76_RS08595 and BWI76_RS08600 are separated by 315 nucleotides
BWI76_RS08580: BWI76_RS08580 - quinolinate synthetase, at 1,810,337 to 1,811,380
_RS08580
BWI76_RS08585: BWI76_RS08585 - nicotinamide riboside transporter PnuC, at 1,811,416 to 1,812,135
_RS08585
BWI76_RS08590: BWI76_RS08590 - zinc transporter ZitB, at 1,812,132 to 1,813,076
_RS08590
BWI76_RS08595: BWI76_RS08595 - hypothetical protein, at 1,813,195 to 1,813,566
_RS08595
BWI76_RS08600: BWI76_RS08600 - 3-deoxy-7-phosphoheptulonate synthase, at 1,813,882 to 1,814,934
_RS08600
Position (kb)
1812
1813
1814 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1811.226 kb on + strand, within BWI76_RS08580 at 1811.471 kb on + strand at 1811.481 kb on + strand at 1811.667 kb on - strand, within BWI76_RS08585 at 1811.805 kb on - strand, within BWI76_RS08585 at 1811.846 kb on + strand, within BWI76_RS08585 at 1812.441 kb on - strand, within BWI76_RS08590 at 1812.443 kb on - strand, within BWI76_RS08590 at 1812.462 kb on - strand, within BWI76_RS08590 at 1812.488 kb on - strand, within BWI76_RS08590 at 1812.512 kb on + strand, within BWI76_RS08590 at 1812.678 kb on - strand, within BWI76_RS08590 at 1812.988 kb on - strand at 1813.082 kb on + strand at 1813.168 kb on - strand at 1813.170 kb on + strand at 1813.178 kb on - strand at 1813.208 kb on - strand at 1813.326 kb on - strand, within BWI76_RS08595 at 1813.337 kb on + strand, within BWI76_RS08595 at 1813.395 kb on - strand, within BWI76_RS08595 at 1813.422 kb on + strand, within BWI76_RS08595 at 1813.454 kb on + strand, within BWI76_RS08595 at 1813.518 kb on + strand, within BWI76_RS08595 at 1813.524 kb on + strand, within BWI76_RS08595 at 1813.634 kb on - strand at 1813.641 kb on + strand at 1813.641 kb on + strand at 1813.721 kb on - strand at 1814.066 kb on - strand, within BWI76_RS08600
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 1,811,226 + BWI76_RS08580 0.85 +1.3 1,811,471 + -0.9 1,811,481 + -0.9 1,811,667 - BWI76_RS08585 0.35 -2.7 1,811,805 - BWI76_RS08585 0.54 +0.0 1,811,846 + BWI76_RS08585 0.60 +1.3 1,812,441 - BWI76_RS08590 0.33 -2.7 1,812,443 - BWI76_RS08590 0.33 -0.9 1,812,462 - BWI76_RS08590 0.35 -1.0 1,812,488 - BWI76_RS08590 0.38 -0.1 1,812,512 + BWI76_RS08590 0.40 -1.3 1,812,678 - BWI76_RS08590 0.58 -1.0 1,812,988 - -2.1 1,813,082 + -2.5 1,813,168 - -1.6 1,813,170 + -1.7 1,813,178 - +0.0 1,813,208 - +0.1 1,813,326 - BWI76_RS08595 0.35 -0.3 1,813,337 + BWI76_RS08595 0.38 +1.0 1,813,395 - BWI76_RS08595 0.54 +0.3 1,813,422 + BWI76_RS08595 0.61 -1.9 1,813,454 + BWI76_RS08595 0.70 -0.1 1,813,518 + BWI76_RS08595 0.87 -2.6 1,813,524 + BWI76_RS08595 0.88 -3.5 1,813,634 - -0.1 1,813,641 + -0.6 1,813,641 + -0.4 1,813,721 - +0.9 1,814,066 - BWI76_RS08600 0.17 +2.6
Or see this region's nucleotide sequence