Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08535 and BWI76_RS08540 are separated by 34 nucleotides BWI76_RS08540 and BWI76_RS08545 are separated by 9 nucleotides BWI76_RS08545 and BWI76_RS08550 are separated by 162 nucleotides BWI76_RS08550 and BWI76_RS08555 are separated by 26 nucleotides BWI76_RS08555 and BWI76_RS08560 are separated by 3 nucleotides BWI76_RS08560 and BWI76_RS08565 are separated by 144 nucleotides BWI76_RS08565 and BWI76_RS08570 are separated by 35 nucleotides
BWI76_RS08535: BWI76_RS08535 - translocation protein TolB, at 1,806,484 to 1,807,776
_RS08535
BWI76_RS08540: BWI76_RS08540 - hypothetical protein, at 1,807,811 to 1,808,335
_RS08540
BWI76_RS08545: BWI76_RS08545 - cell division protein CpoB, at 1,808,345 to 1,809,130
_RS08545
BWI76_RS08550: BWI76_RS08550 - tRNA-Lys, at 1,809,293 to 1,809,368
_RS08550
BWI76_RS08555: BWI76_RS08555 - tRNA-Val, at 1,809,395 to 1,809,470
_RS08555
BWI76_RS08560: BWI76_RS08560 - tRNA-Lys, at 1,809,474 to 1,809,549
_RS08560
BWI76_RS08565: BWI76_RS08565 - tRNA-Lys, at 1,809,694 to 1,809,769
_RS08565
BWI76_RS08570: BWI76_RS08570 - tRNA-Lys, at 1,809,805 to 1,809,880
_RS08570
Position (kb)
1808
1809
1810 Strain fitness (log2 ratio)
-1
0
1 at 1807.741 kb on - strand at 1807.756 kb on + strand at 1808.154 kb on - strand, within BWI76_RS08540 at 1808.166 kb on + strand, within BWI76_RS08540 at 1808.797 kb on + strand, within BWI76_RS08545 at 1808.892 kb on - strand, within BWI76_RS08545 at 1809.582 kb on + strand at 1810.013 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 1,807,741 - +0.6 1,807,756 + +0.1 1,808,154 - BWI76_RS08540 0.65 +1.6 1,808,166 + BWI76_RS08540 0.68 -1.0 1,808,797 + BWI76_RS08545 0.58 -1.7 1,808,892 - BWI76_RS08545 0.70 -0.3 1,809,582 + +1.2 1,810,013 + +0.3
Or see this region's nucleotide sequence