Experiment: KLEB13 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00420 and BWI76_RS00425 overlap by 4 nucleotides BWI76_RS00425 and BWI76_RS00430 are separated by 44 nucleotides BWI76_RS00430 and BWI76_RS00435 are separated by 17 nucleotides
BWI76_RS00420: BWI76_RS00420 - hypothetical protein, at 87,872 to 88,732
_RS00420
BWI76_RS00425: BWI76_RS00425 - D-tyrosyl-tRNA(Tyr) deacylase, at 88,729 to 89,166
_RS00425
BWI76_RS00430: BWI76_RS00430 - GNAT family N-acetyltransferase, at 89,211 to 90,152
_RS00430
BWI76_RS00435: BWI76_RS00435 - lipase, at 90,170 to 91,087
_RS00435
Position (kb) 89
90
91 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 88.295 kb on - strand, within BWI76_RS00420 at 88.316 kb on - strand, within BWI76_RS00420 at 88.464 kb on + strand, within BWI76_RS00420 at 88.464 kb on - strand, within BWI76_RS00420 at 88.472 kb on - strand, within BWI76_RS00420 at 88.484 kb on - strand, within BWI76_RS00420 at 88.523 kb on + strand, within BWI76_RS00420 at 88.531 kb on - strand, within BWI76_RS00420 at 88.599 kb on - strand, within BWI76_RS00420 at 88.648 kb on + strand at 88.736 kb on + strand at 89.025 kb on + strand, within BWI76_RS00425 at 89.025 kb on + strand, within BWI76_RS00425 at 89.046 kb on - strand, within BWI76_RS00425 at 89.216 kb on - strand at 89.390 kb on + strand, within BWI76_RS00430 at 89.453 kb on + strand, within BWI76_RS00430 at 89.461 kb on - strand, within BWI76_RS00430 at 89.638 kb on + strand, within BWI76_RS00430 at 89.817 kb on - strand, within BWI76_RS00430 at 89.870 kb on - strand, within BWI76_RS00430 at 89.872 kb on + strand, within BWI76_RS00430 at 90.050 kb on + strand, within BWI76_RS00430 at 90.280 kb on + strand, within BWI76_RS00435 at 90.282 kb on + strand, within BWI76_RS00435 at 90.301 kb on + strand, within BWI76_RS00435 at 90.323 kb on - strand, within BWI76_RS00435 at 90.477 kb on - strand, within BWI76_RS00435 at 90.560 kb on + strand, within BWI76_RS00435 at 90.578 kb on - strand, within BWI76_RS00435 at 90.585 kb on + strand, within BWI76_RS00435 at 90.611 kb on + strand, within BWI76_RS00435 at 90.619 kb on - strand, within BWI76_RS00435 at 90.641 kb on - strand, within BWI76_RS00435 at 90.670 kb on + strand, within BWI76_RS00435 at 90.805 kb on + strand, within BWI76_RS00435 at 90.813 kb on - strand, within BWI76_RS00435 at 91.063 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB13 MOI 0.1 remove 88,295 - BWI76_RS00420 0.49 -3.1 88,316 - BWI76_RS00420 0.52 -0.5 88,464 + BWI76_RS00420 0.69 +1.4 88,464 - BWI76_RS00420 0.69 -0.8 88,472 - BWI76_RS00420 0.70 +1.0 88,484 - BWI76_RS00420 0.71 +1.5 88,523 + BWI76_RS00420 0.76 -0.5 88,531 - BWI76_RS00420 0.77 -0.3 88,599 - BWI76_RS00420 0.84 +0.3 88,648 + -1.9 88,736 + -2.0 89,025 + BWI76_RS00425 0.68 -1.1 89,025 + BWI76_RS00425 0.68 +0.5 89,046 - BWI76_RS00425 0.72 +0.4 89,216 - +1.6 89,390 + BWI76_RS00430 0.19 +0.4 89,453 + BWI76_RS00430 0.26 -3.3 89,461 - BWI76_RS00430 0.27 -1.9 89,638 + BWI76_RS00430 0.45 -0.6 89,817 - BWI76_RS00430 0.64 -1.1 89,870 - BWI76_RS00430 0.70 -0.6 89,872 + BWI76_RS00430 0.70 -3.2 90,050 + BWI76_RS00430 0.89 +0.1 90,280 + BWI76_RS00435 0.12 +0.8 90,282 + BWI76_RS00435 0.12 -0.2 90,301 + BWI76_RS00435 0.14 +0.9 90,323 - BWI76_RS00435 0.17 +0.7 90,477 - BWI76_RS00435 0.33 +0.1 90,560 + BWI76_RS00435 0.42 +0.1 90,578 - BWI76_RS00435 0.44 +2.2 90,585 + BWI76_RS00435 0.45 -1.0 90,611 + BWI76_RS00435 0.48 +1.2 90,619 - BWI76_RS00435 0.49 +0.5 90,641 - BWI76_RS00435 0.51 +0.3 90,670 + BWI76_RS00435 0.54 +0.3 90,805 + BWI76_RS00435 0.69 -2.0 90,813 - BWI76_RS00435 0.70 +1.1 91,063 + +0.2
Or see this region's nucleotide sequence