Experiment: R2A with Nickel (II) chloride 1696 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS16365 and LRK55_RS16370 are separated by 96 nucleotides LRK55_RS16370 and LRK55_RS16375 are separated by 140 nucleotides LRK55_RS16375 and LRK55_RS16380 are separated by 3 nucleotides LRK55_RS16380 and LRK55_RS16385 overlap by 1 nucleotides
LRK55_RS16365: LRK55_RS16365 - tRNA pseudouridine(13) synthase TruD, at 3,566,944 to 3,567,954
_RS16365
LRK55_RS16370: LRK55_RS16370 - Smr/MutS family protein, at 3,568,051 to 3,568,596
_RS16370
LRK55_RS16375: LRK55_RS16375 - 5'/3'-nucleotidase SurE, at 3,568,737 to 3,569,534
_RS16375
LRK55_RS16380: LRK55_RS16380 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 3,569,538 to 3,570,218
_RS16380
LRK55_RS16385: LRK55_RS16385 - VTT domain-containing protein, at 3,570,218 to 3,570,829
_RS16385
Position (kb)
3568
3569
3570 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3567.913 kb on + strand at 3567.913 kb on + strand at 3567.914 kb on - strand at 3567.914 kb on - strand at 3567.952 kb on + strand at 3567.952 kb on + strand at 3567.953 kb on - strand at 3568.358 kb on + strand, within LRK55_RS16370 at 3568.373 kb on + strand, within LRK55_RS16370 at 3568.373 kb on + strand, within LRK55_RS16370 at 3568.373 kb on + strand, within LRK55_RS16370 at 3568.373 kb on + strand, within LRK55_RS16370 at 3568.374 kb on - strand, within LRK55_RS16370 at 3568.374 kb on - strand, within LRK55_RS16370 at 3568.374 kb on - strand, within LRK55_RS16370 at 3568.374 kb on - strand, within LRK55_RS16370 at 3568.607 kb on + strand at 3568.607 kb on + strand at 3568.607 kb on + strand at 3568.607 kb on + strand at 3568.607 kb on + strand at 3568.607 kb on + strand at 3568.607 kb on + strand at 3568.607 kb on + strand at 3568.608 kb on - strand at 3568.608 kb on - strand at 3568.608 kb on - strand at 3568.608 kb on - strand at 3568.608 kb on - strand at 3568.608 kb on - strand at 3568.608 kb on - strand at 3568.608 kb on - strand at 3568.608 kb on - strand at 3568.914 kb on + strand, within LRK55_RS16375 at 3568.914 kb on + strand, within LRK55_RS16375 at 3568.914 kb on + strand, within LRK55_RS16375 at 3568.914 kb on + strand, within LRK55_RS16375 at 3569.040 kb on + strand, within LRK55_RS16375 at 3569.040 kb on + strand, within LRK55_RS16375 at 3569.040 kb on + strand, within LRK55_RS16375 at 3569.041 kb on - strand, within LRK55_RS16375 at 3569.041 kb on - strand, within LRK55_RS16375 at 3569.136 kb on + strand, within LRK55_RS16375 at 3569.136 kb on + strand, within LRK55_RS16375 at 3569.136 kb on + strand, within LRK55_RS16375 at 3569.136 kb on + strand, within LRK55_RS16375 at 3569.136 kb on + strand, within LRK55_RS16375 at 3569.137 kb on - strand, within LRK55_RS16375 at 3569.137 kb on - strand, within LRK55_RS16375 at 3569.547 kb on + strand at 3569.547 kb on + strand at 3569.547 kb on + strand at 3569.547 kb on + strand at 3569.547 kb on + strand at 3569.547 kb on + strand at 3569.548 kb on - strand at 3569.548 kb on - strand at 3569.548 kb on - strand at 3569.718 kb on + strand, within LRK55_RS16380 at 3569.719 kb on - strand, within LRK55_RS16380 at 3569.974 kb on - strand, within LRK55_RS16380 at 3570.239 kb on + strand at 3570.239 kb on + strand at 3570.239 kb on + strand at 3570.239 kb on + strand at 3570.239 kb on + strand at 3570.240 kb on - strand at 3570.240 kb on - strand at 3570.240 kb on - strand at 3570.240 kb on - strand at 3570.240 kb on - strand at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.281 kb on + strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.282 kb on - strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.284 kb on + strand, within LRK55_RS16385 at 3570.285 kb on - strand, within LRK55_RS16385 at 3570.285 kb on - strand, within LRK55_RS16385 at 3570.285 kb on - strand, within LRK55_RS16385 at 3570.285 kb on - strand, within LRK55_RS16385 at 3570.285 kb on - strand, within LRK55_RS16385 at 3570.285 kb on - strand, within LRK55_RS16385 at 3570.285 kb on - strand, within LRK55_RS16385 at 3570.285 kb on - strand, within LRK55_RS16385 at 3570.434 kb on + strand, within LRK55_RS16385 at 3570.434 kb on + strand, within LRK55_RS16385 at 3570.434 kb on + strand, within LRK55_RS16385 at 3570.434 kb on + strand, within LRK55_RS16385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 1696 uM remove 3,567,913 + +1.1 3,567,913 + +0.5 3,567,914 - +0.6 3,567,914 - -0.1 3,567,952 + +0.2 3,567,952 + -0.4 3,567,953 - +0.4 3,568,358 + LRK55_RS16370 0.56 -0.8 3,568,373 + LRK55_RS16370 0.59 -1.4 3,568,373 + LRK55_RS16370 0.59 +0.0 3,568,373 + LRK55_RS16370 0.59 +0.1 3,568,373 + LRK55_RS16370 0.59 +0.9 3,568,374 - LRK55_RS16370 0.59 -0.1 3,568,374 - LRK55_RS16370 0.59 +0.7 3,568,374 - LRK55_RS16370 0.59 -0.2 3,568,374 - LRK55_RS16370 0.59 +0.2 3,568,607 + -2.0 3,568,607 + -0.1 3,568,607 + -0.2 3,568,607 + +0.3 3,568,607 + -1.2 3,568,607 + -1.2 3,568,607 + +0.5 3,568,607 + -2.2 3,568,608 - -1.9 3,568,608 - -0.6 3,568,608 - -1.8 3,568,608 - -0.4 3,568,608 - +0.1 3,568,608 - +0.1 3,568,608 - +0.3 3,568,608 - -2.7 3,568,608 - +0.1 3,568,914 + LRK55_RS16375 0.22 +0.6 3,568,914 + LRK55_RS16375 0.22 -0.0 3,568,914 + LRK55_RS16375 0.22 +0.4 3,568,914 + LRK55_RS16375 0.22 +0.1 3,569,040 + LRK55_RS16375 0.38 -2.5 3,569,040 + LRK55_RS16375 0.38 -0.7 3,569,040 + LRK55_RS16375 0.38 +0.4 3,569,041 - LRK55_RS16375 0.38 -1.4 3,569,041 - LRK55_RS16375 0.38 -1.6 3,569,136 + LRK55_RS16375 0.50 -2.2 3,569,136 + LRK55_RS16375 0.50 -1.6 3,569,136 + LRK55_RS16375 0.50 -0.1 3,569,136 + LRK55_RS16375 0.50 +0.4 3,569,136 + LRK55_RS16375 0.50 +2.0 3,569,137 - LRK55_RS16375 0.50 -3.4 3,569,137 - LRK55_RS16375 0.50 -1.4 3,569,547 + -1.2 3,569,547 + +0.1 3,569,547 + -1.0 3,569,547 + -1.2 3,569,547 + -1.4 3,569,547 + -0.4 3,569,548 - -0.4 3,569,548 - -2.6 3,569,548 - -1.2 3,569,718 + LRK55_RS16380 0.26 -1.5 3,569,719 - LRK55_RS16380 0.27 -1.4 3,569,974 - LRK55_RS16380 0.64 -1.4 3,570,239 + -2.3 3,570,239 + -0.8 3,570,239 + -1.4 3,570,239 + -1.3 3,570,239 + -3.3 3,570,240 - -2.2 3,570,240 - -1.2 3,570,240 - -0.4 3,570,240 - -2.3 3,570,240 - -1.8 3,570,281 + LRK55_RS16385 0.10 -1.8 3,570,281 + LRK55_RS16385 0.10 -2.2 3,570,281 + LRK55_RS16385 0.10 -1.7 3,570,281 + LRK55_RS16385 0.10 -2.4 3,570,281 + LRK55_RS16385 0.10 -1.4 3,570,281 + LRK55_RS16385 0.10 -2.1 3,570,281 + LRK55_RS16385 0.10 -1.3 3,570,281 + LRK55_RS16385 0.10 -1.6 3,570,281 + LRK55_RS16385 0.10 -3.5 3,570,281 + LRK55_RS16385 0.10 -1.6 3,570,281 + LRK55_RS16385 0.10 -0.8 3,570,281 + LRK55_RS16385 0.10 -1.6 3,570,281 + LRK55_RS16385 0.10 -1.6 3,570,282 - LRK55_RS16385 0.10 -2.6 3,570,282 - LRK55_RS16385 0.10 -2.2 3,570,282 - LRK55_RS16385 0.10 -2.6 3,570,282 - LRK55_RS16385 0.10 -1.4 3,570,282 - LRK55_RS16385 0.10 -3.2 3,570,282 - LRK55_RS16385 0.10 -1.6 3,570,282 - LRK55_RS16385 0.10 -3.9 3,570,282 - LRK55_RS16385 0.10 -1.4 3,570,282 - LRK55_RS16385 0.10 -1.4 3,570,282 - LRK55_RS16385 0.10 -2.2 3,570,282 - LRK55_RS16385 0.10 -2.3 3,570,284 + LRK55_RS16385 0.11 -1.4 3,570,284 + LRK55_RS16385 0.11 -2.7 3,570,284 + LRK55_RS16385 0.11 -3.1 3,570,284 + LRK55_RS16385 0.11 -1.1 3,570,284 + LRK55_RS16385 0.11 -4.0 3,570,284 + LRK55_RS16385 0.11 -0.8 3,570,284 + LRK55_RS16385 0.11 -1.0 3,570,284 + LRK55_RS16385 0.11 -2.9 3,570,284 + LRK55_RS16385 0.11 -2.4 3,570,284 + LRK55_RS16385 0.11 -2.9 3,570,284 + LRK55_RS16385 0.11 -2.5 3,570,285 - LRK55_RS16385 0.11 -1.4 3,570,285 - LRK55_RS16385 0.11 -2.0 3,570,285 - LRK55_RS16385 0.11 -0.8 3,570,285 - LRK55_RS16385 0.11 -2.0 3,570,285 - LRK55_RS16385 0.11 -3.0 3,570,285 - LRK55_RS16385 0.11 -2.5 3,570,285 - LRK55_RS16385 0.11 -3.3 3,570,285 - LRK55_RS16385 0.11 -2.0 3,570,434 + LRK55_RS16385 0.35 -2.0 3,570,434 + LRK55_RS16385 0.35 -2.9 3,570,434 + LRK55_RS16385 0.35 -1.6 3,570,434 + LRK55_RS16385 0.35 +0.2
Or see this region's nucleotide sequence