Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2216

Experiment: L-Valine 10 mM (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2215 and Rv2216 overlap by 1 nucleotidesRv2216 and Rv2217 are separated by 52 nucleotidesRv2217 and Rv2218 overlap by 4 nucleotides Rv2215: Rv2215 - DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase, at 2,481,965 to 2,483,626 Rv2215 Rv2216: Rv2216 - Conserved protein, at 2,483,626 to 2,484,531 Rv2216 Rv2217: Rv2217 - Probable lipoate biosynthesis protein B LipB, at 2,484,584 to 2,485,276 Rv2217 Rv2218: Rv2218 - Probable lipoate biosynthesis protein A LipA, at 2,485,273 to 2,486,208 Rv2218 Position (kb) 2483 2484 2485Strain fitness (log2 ratio) -1 0 1at 2484.205 kb on + strand, within Rv2216at 2484.205 kb on + strand, within Rv2216at 2484.205 kb on + strand, within Rv2216at 2484.206 kb on - strand, within Rv2216at 2484.206 kb on - strand, within Rv2216at 2484.207 kb on + strand, within Rv2216at 2484.207 kb on + strand, within Rv2216at 2484.207 kb on + strand, within Rv2216at 2484.207 kb on + strand, within Rv2216at 2484.572 kb on - strandat 2484.580 kb on + strandat 2484.580 kb on + strandat 2485.261 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine 10 mM (N)
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2,484,205 + Rv2216 0.64 -0.5
2,484,205 + Rv2216 0.64 -0.6
2,484,205 + Rv2216 0.64 -0.9
2,484,206 - Rv2216 0.64 +0.0
2,484,206 - Rv2216 0.64 -0.2
2,484,207 + Rv2216 0.64 -0.6
2,484,207 + Rv2216 0.64 +0.7
2,484,207 + Rv2216 0.64 -1.0
2,484,207 + Rv2216 0.64 -0.7
2,484,572 - -0.7
2,484,580 + +0.7
2,484,580 + -0.6
2,485,261 - +0.5

Or see this region's nucleotide sequence