Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2088

Experiment: Glycine 10 mM (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2086 and Rv2087 are separated by 77 nucleotidesRv2087 and Rv2088 are separated by 186 nucleotidesRv2088 and Rv2089c are separated by 16 nucleotides Rv2086: Rv2086 - Conserved hypothetical protein, at 2,343,311 to 2,343,916 Rv2086 Rv2087: Rv2087 - Conserved hypothetical protein, at 2,343,994 to 2,344,224 Rv2087 Rv2088: Rv2088 - Transmembrane serine/threonine-protein kinase J PknJ (protein kinase J) (STPK J), at 2,344,411 to 2,346,180 Rv2088 Rv2089c: Rv2089c - Dipeptidase PepE, at 2,346,197 to 2,347,324 Rv2089c Position (kb) 2344 2345 2346 2347Strain fitness (log2 ratio) -1 0 1at 2343.506 kb on + strand, within Rv2086at 2343.548 kb on + strand, within Rv2086at 2343.736 kb on + strand, within Rv2086at 2343.915 kb on - strandat 2343.915 kb on - strandat 2344.053 kb on - strand, within Rv2087at 2344.053 kb on - strand, within Rv2087at 2344.053 kb on - strand, within Rv2087at 2344.256 kb on + strandat 2344.257 kb on - strandat 2344.257 kb on - strandat 2344.262 kb on - strandat 2344.262 kb on - strandat 2344.262 kb on - strandat 2344.333 kb on + strandat 2344.334 kb on - strandat 2344.493 kb on + strandat 2344.493 kb on + strandat 2344.494 kb on - strandat 2344.495 kb on + strandat 2344.753 kb on + strand, within Rv2088at 2346.078 kb on + strandat 2346.155 kb on - strandat 2346.171 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Glycine 10 mM (N)
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2,343,506 + Rv2086 0.32 +0.3
2,343,548 + Rv2086 0.39 -0.3
2,343,736 + Rv2086 0.70 +0.0
2,343,915 - -0.2
2,343,915 - -1.0
2,344,053 - Rv2087 0.26 -0.5
2,344,053 - Rv2087 0.26 -0.9
2,344,053 - Rv2087 0.26 +0.4
2,344,256 + +0.3
2,344,257 - +1.7
2,344,257 - -0.5
2,344,262 - +0.1
2,344,262 - +0.2
2,344,262 - +0.4
2,344,333 + +0.7
2,344,334 - +0.7
2,344,493 + +0.5
2,344,493 + -0.4
2,344,494 - -0.1
2,344,495 + +0.0
2,344,753 + Rv2088 0.19 -0.8
2,346,078 + +1.7
2,346,155 - +0.3
2,346,171 - +0.2

Or see this region's nucleotide sequence