Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0554

Experiment: L-Glutamic 10 mM (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0552 and Rv0553 overlap by 4 nucleotidesRv0553 and Rv0554 overlap by 4 nucleotidesRv0554 and Rv0555 are separated by 42 nucleotides Rv0552: Rv0552 - Conserved protein, at 642,889 to 644,493 Rv0552 Rv0553: Rv0553 - Probable muconate cycloisomerase MenC (cis,cis-muconate lactonizing enzyme) (MLE), at 644,490 to 645,470 Rv0553 Rv0554: Rv0554 - Possible peroxidase BpoC (non-haem peroxidase), at 645,467 to 646,255 Rv0554 Rv0555: Rv0555 - Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha-ketoglutarate decarboxylase) (KDC), at 646,298 to 647,962 Rv0555 Position (kb) 645 646 647Strain fitness (log2 ratio) -1 0 1at 645.694 kb on - strand, within Rv0554at 645.694 kb on - strand, within Rv0554at 645.857 kb on + strand, within Rv0554at 645.858 kb on - strand, within Rv0554at 645.887 kb on + strand, within Rv0554at 645.887 kb on + strand, within Rv0554at 645.887 kb on + strand, within Rv0554at 645.906 kb on + strand, within Rv0554at 645.907 kb on - strand, within Rv0554at 645.989 kb on - strand, within Rv0554at 645.989 kb on - strand, within Rv0554at 646.018 kb on - strand, within Rv0554at 646.116 kb on - strand, within Rv0554at 646.293 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamic 10 mM (N)
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645,694 - Rv0554 0.29 +0.4
645,694 - Rv0554 0.29 +1.0
645,857 + Rv0554 0.49 +1.3
645,858 - Rv0554 0.50 +1.0
645,887 + Rv0554 0.53 +0.5
645,887 + Rv0554 0.53 +1.2
645,887 + Rv0554 0.53 +0.8
645,906 + Rv0554 0.56 +1.1
645,907 - Rv0554 0.56 +0.9
645,989 - Rv0554 0.66 +1.1
645,989 - Rv0554 0.66 +0.9
646,018 - Rv0554 0.70 +1.2
646,116 - Rv0554 0.82 +1.0
646,293 + -0.1

Or see this region's nucleotide sequence