Experiment: L-Glutamic 10 mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0552 and Rv0553 overlap by 4 nucleotides Rv0553 and Rv0554 overlap by 4 nucleotides Rv0554 and Rv0555 are separated by 42 nucleotides
Rv0552: Rv0552 - Conserved protein, at 642,889 to 644,493
Rv0552
Rv0553: Rv0553 - Probable muconate cycloisomerase MenC (cis,cis-muconate lactonizing enzyme) (MLE), at 644,490 to 645,470
Rv0553
Rv0554: Rv0554 - Possible peroxidase BpoC (non-haem peroxidase), at 645,467 to 646,255
Rv0554
Rv0555: Rv0555 - Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha-ketoglutarate decarboxylase) (KDC), at 646,298 to 647,962
Rv0555
Position (kb)
645
646
647 Strain fitness (log2 ratio)
-1
0
1 at 645.694 kb on - strand, within Rv0554 at 645.694 kb on - strand, within Rv0554 at 645.857 kb on + strand, within Rv0554 at 645.858 kb on - strand, within Rv0554 at 645.887 kb on + strand, within Rv0554 at 645.887 kb on + strand, within Rv0554 at 645.887 kb on + strand, within Rv0554 at 645.906 kb on + strand, within Rv0554 at 645.907 kb on - strand, within Rv0554 at 645.989 kb on - strand, within Rv0554 at 645.989 kb on - strand, within Rv0554 at 646.018 kb on - strand, within Rv0554 at 646.116 kb on - strand, within Rv0554 at 646.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic 10 mM (N) remove 645,694 - Rv0554 0.29 +0.4 645,694 - Rv0554 0.29 +1.0 645,857 + Rv0554 0.49 +1.3 645,858 - Rv0554 0.50 +1.0 645,887 + Rv0554 0.53 +0.5 645,887 + Rv0554 0.53 +1.2 645,887 + Rv0554 0.53 +0.8 645,906 + Rv0554 0.56 +1.1 645,907 - Rv0554 0.56 +0.9 645,989 - Rv0554 0.66 +1.1 645,989 - Rv0554 0.66 +0.9 646,018 - Rv0554 0.70 +1.2 646,116 - Rv0554 0.82 +1.0 646,293 + -0.1
Or see this region's nucleotide sequence