Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3335c

Experiment: L-Asparagine 10 mM (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3333c and Rv3334 are separated by 474 nucleotidesRv3334 and Rv3335c are separated by 33 nucleotidesRv3335c and Rv3336c are separated by 20 nucleotides Rv3333c: Rv3333c - Hypothetical proline rich protein, at 3,719,937 to 3,720,782 Rv3333c Rv3334: Rv3334 - Probable transcriptional regulatory protein (probably MerR-family), at 3,721,257 to 3,721,697 Rv3334 Rv3335c: Rv3335c - Probable conserved integral membrane protein, at 3,721,731 to 3,722,600 Rv3335c Rv3336c: Rv3336c - Probable tryptophanyl-tRNA synthetase TrpS (tryptophan--tRNA ligase) (TRPRS) (tryptophan translase), at 3,722,621 to 3,723,631 Rv3336c Position (kb) 3721 3722 3723Strain fitness (log2 ratio) -1 0 1 2at 3720.803 kb on - strandat 3720.845 kb on + strandat 3720.845 kb on + strandat 3720.845 kb on + strandat 3721.054 kb on + strandat 3721.055 kb on - strandat 3721.121 kb on + strandat 3721.122 kb on - strandat 3721.210 kb on + strandat 3721.211 kb on - strandat 3721.211 kb on - strandat 3721.315 kb on - strand, within Rv3334at 3721.371 kb on + strand, within Rv3334at 3721.404 kb on - strand, within Rv3334at 3721.597 kb on - strand, within Rv3334at 3721.851 kb on + strand, within Rv3335cat 3721.862 kb on - strand, within Rv3335cat 3722.154 kb on + strand, within Rv3335cat 3722.471 kb on - strand, within Rv3335cat 3722.622 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine 10 mM (N)
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3,720,803 - +0.5
3,720,845 + -0.5
3,720,845 + -0.7
3,720,845 + -0.0
3,721,054 + -0.1
3,721,055 - +1.6
3,721,121 + -0.1
3,721,122 - -0.0
3,721,210 + +0.0
3,721,211 - -0.1
3,721,211 - +1.5
3,721,315 - Rv3334 0.13 +0.8
3,721,371 + Rv3334 0.26 +2.5
3,721,404 - Rv3334 0.33 -0.2
3,721,597 - Rv3334 0.77 +0.0
3,721,851 + Rv3335c 0.14 +2.2
3,721,862 - Rv3335c 0.15 -0.2
3,722,154 + Rv3335c 0.49 -0.2
3,722,471 - Rv3335c 0.85 -1.1
3,722,622 + -0.4

Or see this region's nucleotide sequence