Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2681

Experiment: L-Asparagine 10 mM (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2679 and Rv2680 are separated by 159 nucleotidesRv2680 and Rv2681 are separated by 1 nucleotidesRv2681 and Rv2682c overlap by 4 nucleotides Rv2679: Rv2679 - Probable enoyl-CoA hydratase EchA15 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 2,995,115 to 2,995,945 Rv2679 Rv2680: Rv2680 - Conserved protein, at 2,996,105 to 2,996,737 Rv2680 Rv2681: Rv2681 - Conserved hypothetical alanine rich protein, at 2,996,739 to 2,998,055 Rv2681 Rv2682c: Rv2682c - Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs1 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS), at 2,998,052 to 2,999,968 Rv2682c Position (kb) 2996 2997 2998 2999Strain fitness (log2 ratio) -1 0 1 2at 2995.797 kb on - strand, within Rv2679at 2995.989 kb on + strandat 2996.277 kb on - strand, within Rv2680at 2996.367 kb on - strand, within Rv2680at 2996.402 kb on - strand, within Rv2680at 2996.417 kb on + strand, within Rv2680at 2996.875 kb on + strand, within Rv2681at 2996.987 kb on - strand, within Rv2681at 2997.116 kb on + strand, within Rv2681at 2997.133 kb on + strand, within Rv2681at 2997.135 kb on + strand, within Rv2681at 2997.136 kb on - strand, within Rv2681at 2997.136 kb on - strand, within Rv2681at 2997.206 kb on - strand, within Rv2681at 2997.283 kb on - strand, within Rv2681at 2997.576 kb on - strand, within Rv2681at 2997.576 kb on - strand, within Rv2681at 2997.942 kb on + strandat 2997.943 kb on - strandat 2997.982 kb on + strandat 2997.982 kb on + strandat 2997.982 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine 10 mM (N)
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2,995,797 - Rv2679 0.82 -1.4
2,995,989 + -0.7
2,996,277 - Rv2680 0.27 -0.4
2,996,367 - Rv2680 0.41 -1.3
2,996,402 - Rv2680 0.47 +0.9
2,996,417 + Rv2680 0.49 +1.9
2,996,875 + Rv2681 0.10 +1.3
2,996,987 - Rv2681 0.19 +0.5
2,997,116 + Rv2681 0.29 +0.1
2,997,133 + Rv2681 0.30 +1.9
2,997,135 + Rv2681 0.30 +0.6
2,997,136 - Rv2681 0.30 +0.8
2,997,136 - Rv2681 0.30 +1.4
2,997,206 - Rv2681 0.35 +1.1
2,997,283 - Rv2681 0.41 +1.0
2,997,576 - Rv2681 0.64 +0.4
2,997,576 - Rv2681 0.64 +0.5
2,997,942 + +0.5
2,997,943 - +1.2
2,997,982 + +0.1
2,997,982 + +0.8
2,997,982 + +0.4

Or see this region's nucleotide sequence