Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2614c

Experiment: L-Asparagine 10 mM (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2612c and Rv2613c overlap by 4 nucleotidesRv2613c and Rv2614c overlap by 8 nucleotidesRv2614c and Rv2614A are separated by 108 nucleotidesRv2614A and Rv2615c overlap by 4 nucleotides Rv2612c: Rv2612c - PI synthase PgsA1 (phosphatidylinositol synthase) (CDP-diacylglycerol--inositol-3-phosphatidyltransferase), at 2,939,959 to 2,940,612 Rv2612c Rv2613c: Rv2613c - Conserved protein, at 2,940,609 to 2,941,196 Rv2613c Rv2614c: Rv2614c - Probable threonyl-tRNA synthetase ThrS (threonine-tRNA synthetase)(ThrRS) (threonine-tRNA ligase), at 2,941,189 to 2,943,267 Rv2614c Rv2614A: Rv2614A - Conserved hypothetical protein, at 2,943,376 to 2,943,603 Rv2614A Rv2615c: Rv2615c - PE-PGRS family protein PE_PGRS45, at 2,943,600 to 2,944,985 Rv2615c Position (kb) 2941 2942 2943 2944Strain fitness (log2 ratio) -1 0 1at 2940.778 kb on - strand, within Rv2613cat 2940.778 kb on - strand, within Rv2613cat 2940.938 kb on - strand, within Rv2613cat 2941.085 kb on - strand, within Rv2613cat 2941.085 kb on - strand, within Rv2613cat 2941.225 kb on - strandat 2943.340 kb on + strandat 2943.341 kb on - strandat 2943.923 kb on + strand, within Rv2615cat 2943.979 kb on + strand, within Rv2615cat 2943.980 kb on - strand, within Rv2615cat 2943.980 kb on - strand, within Rv2615cat 2943.980 kb on - strand, within Rv2615c

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine 10 mM (N)
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2,940,778 - Rv2613c 0.29 -0.1
2,940,778 - Rv2613c 0.29 +0.4
2,940,938 - Rv2613c 0.56 -1.7
2,941,085 - Rv2613c 0.81 -0.3
2,941,085 - Rv2613c 0.81 -0.8
2,941,225 - +0.9
2,943,340 + +0.1
2,943,341 - -0.9
2,943,923 + Rv2615c 0.23 +0.5
2,943,979 + Rv2615c 0.27 +1.3
2,943,980 - Rv2615c 0.27 -0.3
2,943,980 - Rv2615c 0.27 +0.5
2,943,980 - Rv2615c 0.27 +0.1

Or see this region's nucleotide sequence