Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2065

Experiment: Palmitic 100 uM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2064 and Rv2065 are separated by 9 nucleotidesRv2065 and Rv2066 overlap by 4 nucleotides Rv2064: Rv2064 - Precorrin-3B synthase CobG, at 2,321,451 to 2,322,542 Rv2064 Rv2065: Rv2065 - Precorrin-8X methylmutase CobH (aka precorrin isomerase), at 2,322,552 to 2,323,178 Rv2065 Rv2066: Rv2066 - Probable bifunctional protein, CobI-COBJ fusion protein: S-adenosyl-L-methionine-precorrin-2 methyl transferase + precorrin-3 methylase, at 2,323,175 to 2,324,701 Rv2066 Position (kb) 2322 2323 2324Strain fitness (log2 ratio) -2 -1 0 1 2at 2321.729 kb on + strand, within Rv2064at 2322.041 kb on - strand, within Rv2064at 2322.566 kb on - strandat 2322.566 kb on - strandat 2322.566 kb on - strandat 2322.949 kb on + strand, within Rv2065at 2322.950 kb on - strand, within Rv2065at 2323.098 kb on + strand, within Rv2065at 2323.099 kb on - strand, within Rv2065at 2323.099 kb on - strand, within Rv2065at 2323.281 kb on - strandat 2323.331 kb on + strand, within Rv2066at 2323.331 kb on + strand, within Rv2066at 2323.431 kb on - strand, within Rv2066at 2323.431 kb on - strand, within Rv2066at 2323.514 kb on + strand, within Rv2066at 2323.515 kb on - strand, within Rv2066at 2323.515 kb on - strand, within Rv2066at 2323.515 kb on - strand, within Rv2066at 2323.523 kb on + strand, within Rv2066at 2323.523 kb on + strand, within Rv2066at 2323.523 kb on + strand, within Rv2066at 2323.524 kb on - strand, within Rv2066at 2323.524 kb on - strand, within Rv2066at 2323.524 kb on - strand, within Rv2066at 2323.862 kb on + strand, within Rv2066at 2323.863 kb on - strand, within Rv2066at 2323.863 kb on - strand, within Rv2066at 2323.863 kb on - strand, within Rv2066at 2324.161 kb on - strand, within Rv2066at 2324.161 kb on - strand, within Rv2066

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Per-strain Table

Position Strand Gene LocusTag Fraction Palmitic 100 uM (C)
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2,321,729 + Rv2064 0.25 +0.2
2,322,041 - Rv2064 0.54 +2.2
2,322,566 - -0.0
2,322,566 - -0.5
2,322,566 - +1.0
2,322,949 + Rv2065 0.63 +0.6
2,322,950 - Rv2065 0.63 +1.1
2,323,098 + Rv2065 0.87 -1.1
2,323,099 - Rv2065 0.87 +0.1
2,323,099 - Rv2065 0.87 +0.8
2,323,281 - -1.1
2,323,331 + Rv2066 0.10 +0.4
2,323,331 + Rv2066 0.10 -0.5
2,323,431 - Rv2066 0.17 -1.0
2,323,431 - Rv2066 0.17 +0.4
2,323,514 + Rv2066 0.22 -0.3
2,323,515 - Rv2066 0.22 -0.3
2,323,515 - Rv2066 0.22 -0.2
2,323,515 - Rv2066 0.22 -0.2
2,323,523 + Rv2066 0.23 -1.8
2,323,523 + Rv2066 0.23 -0.4
2,323,523 + Rv2066 0.23 -0.0
2,323,524 - Rv2066 0.23 +1.8
2,323,524 - Rv2066 0.23 +0.9
2,323,524 - Rv2066 0.23 -0.5
2,323,862 + Rv2066 0.45 +0.2
2,323,863 - Rv2066 0.45 -0.1
2,323,863 - Rv2066 0.45 +1.5
2,323,863 - Rv2066 0.45 -0.5
2,324,161 - Rv2066 0.65 -0.2
2,324,161 - Rv2066 0.65 -0.1

Or see this region's nucleotide sequence