Experiment: Stearic 100 uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3646c and Rv3647c are separated by 352 nucleotides Rv3647c and Rv3648c are separated by 139 nucleotides Rv3648c and Rv3649 are separated by 249 nucleotides
Rv3646c: Rv3646c - DNA topoisomerase I TopA (omega-protein) (relaxing enzyme) (untwisting enzyme) (swivelase) (type I DNA topoisomerase) (nicking-closing enzyme) (TOPO I), at 4,084,453 to 4,087,257
Rv3646c
Rv3647c: Rv3647c - Conserved hypothetical protein, at 4,087,610 to 4,088,188
Rv3647c
Rv3648c: Rv3648c - Probable cold shock protein A CspA, at 4,088,328 to 4,088,531
Rv3648c
Rv3649: Rv3649 - Probable helicase, at 4,088,781 to 4,091,096
Rv3649
Position (kb)
4087
4088
4089 Strain fitness (log2 ratio)
-1
0
1 at 4087.400 kb on - strand at 4087.497 kb on + strand at 4087.498 kb on - strand at 4087.498 kb on - strand at 4087.628 kb on + strand at 4087.628 kb on + strand at 4087.628 kb on + strand at 4087.629 kb on - strand at 4087.629 kb on - strand at 4088.070 kb on - strand, within Rv3647c at 4088.154 kb on - strand at 4088.154 kb on - strand at 4088.227 kb on - strand at 4088.270 kb on + strand at 4088.270 kb on + strand at 4088.271 kb on - strand at 4088.271 kb on - strand at 4088.766 kb on - strand at 4088.766 kb on - strand at 4088.991 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stearic 100 uM (C) remove 4,087,400 - +0.0 4,087,497 + -0.8 4,087,498 - -0.0 4,087,498 - +0.9 4,087,628 + -0.4 4,087,628 + -0.5 4,087,628 + -0.2 4,087,629 - -0.4 4,087,629 - +0.1 4,088,070 - Rv3647c 0.79 +0.2 4,088,154 - +0.4 4,088,154 - +1.4 4,088,227 - +1.2 4,088,270 + -0.1 4,088,270 + -0.1 4,088,271 - -0.6 4,088,271 - -0.1 4,088,766 - +0.4 4,088,766 - +0.1 4,088,991 + -1.1
Or see this region's nucleotide sequence